摘要
目的:探讨腺苷对癫痫大鼠发作行为、脑电图(EEG)及海马缝隙连接蛋白43(CX43)的影响。方法:雄性Wistar大鼠随机分成对照组、红藻氨酸(KA)组及腺苷组。采用视频监视观察2周和5周大鼠癫痫发作情况以及EEG,应用免疫印迹法检测CX43的表达。结果:2周时腺苷组大鼠行为学发作级别、EEG与KA组无明显差异,5周时腺苷组行为学发作级别明显降低,EEG癫痫放电频率降低。对照组无发作,EEG无癫痫波。KA和腺苷组大鼠在2周和5周时CX43表达均明显增加;5周时腺苷组CX43表达水平显著低与KA组。结论:CX43在癫痫大鼠海马组织中的表达变化与癫痫发作相关;腺苷可以明显减轻大鼠癫痫发作级别,降低癫痫放电频率。腺苷可能通过抑制KA诱导的大鼠癫痫发作时CX43的表达而发挥抗癫痫作用。
Objective： To discuss the effect of adenosine on kainate（KA）-induced seizures, EEG and the expression of gap junction protein connexin 43 （CX43） in rats with seizure. Methods： Wistar rats were randomly divided into three groups： control group （KA） group and adenosine group. Seizure situation and EEG were observed 2 weeks and 5 weeks after vector injection. Expression of CX43 was detected by Western blotting. Results： Compared with KA group, scale of seizure and EEG in rats of adenosine group had no significant difference at week 2, but was significantly reduced at week 5 and EEG epileptic discharge frequency was reduced. Control group had no seizures. Compared with the control group, the expression of protein CX43 in KA group and adenosine group significantly increased at week 2 and at week 5, and expression of CX43 in the adenosine group at week 5 significantly decreased compared with the KA group. Conclusion： Adenosine reduces scale of seizure in KA-induced rat model. Adenosine may produce anti-epileptic and neuroprotective effects by inhibiting the expression of gap junction protein in the hippocampus of KA-induced epileptic rat model.
出处
《解剖学杂志》
CSCD
北大核心
2017年第6期689-691,697,共4页
Chinese Journal of Anatomy
