摘要
目的通过测定5-氨基酮戊酸(5-ALA)与白念珠菌菌悬液不同孵育时间生成Pp IX的水平及5-氨基酮戊酸光动力疗法(5-ALA-PDT)抑菌率,为临床选择最佳孵育时间提供理论依据。方法制备白念珠菌悬液并与ALA避光孵育,设实验组和对照组。采用激光共聚焦显微镜观察Pp IX产生情况,MTT法测定ALA光动力治疗对白念珠菌生长的抑制率。结果白念珠菌菌悬液与ALA避光孵育后有荧光物质Pp IX产生,孵育30 min到90 min产生的Pp IX最多,120 min以后Pp IX开始明显减少,对照组无荧光物质出现。结论 ALA-PDT对白念珠菌抑制效应同孵育时间密切相关。这为临床ALA-PDT治疗白念珠菌疾病提供了理论依据。
Objective To facilitate the selection of the best lighting time by determining the level at which C.albicans and 5-ALA PDT generate protoporphyrin IX（ Pp IX） at different time points and by measuring the bacteriostatic rate of 5-amino ketones pentanoic acid photodynamic therapy（ 5-ALA PDT）.Method C.albicans suspension was prepared and incubated with ALA in the dark.The consequent mixture was divided into the control group and experimental group. The generation of Pp IX was observed under a laser confocal microscope.The inhibitory rate of 5-ALA PDT on C.albicans was determined by MTT method.Result After dark incubation,C. albicans and ALA produced fluorescent Pp IX that reached the peak amount between 30 min and 90 min,but began to decrease significantly at 120 min.There was no fluorescent material in control group. Conclusion The inhibitory effect of ALA PDT on C. albicans was closely related to the concentration of the photosensitizer,which provides data for treatment of C.albicans disease.
出处
《中国真菌学杂志》
CSCD
2015年第3期146-150,共5页
Chinese Journal of Mycology
关键词
5-氨基酮戊酸
光动力
白念珠菌
孵育时间
5-amino ketones pentanoic acid
photodynamic
C.albicans
drug concentration
