摘要
目的探讨局部应用人脂联素(h APN)基因重组腺病毒Ad-h APN-EGFP对SD大鼠胫骨骨缺损修复的影响。方法采用36只SD大鼠进行胫骨骨缺损(缺损直径2 mm)修复实验,36只大鼠(72侧)随机分为A、B、C共3组(n=24),在术中及术后第2天分别注射重组腺病毒Ad-h APN-EGFP、空白腺病毒Ad-EGFP和生理盐水。术后1周采用实时荧光定量聚合酶链反应(real-time PCR)对h APN及成骨相关因子的表达进行检测,术后3周采用显微CT、苏木精-伊红(HE)染色及Masson染色对骨缺损修复情况进行对比。结果 1)术后1周real-time PCR显示,A组h APN表达成功,而B、C组无表达;A组成骨相关因子的表达量明显高于B、C组(P<0.05)。2)术后3周显微CT、HE染色及Masson染色显示,A组新生骨密度、骨体积分数、骨小梁数量及骨小梁厚度均大于B、C组(P<0.05),而B、C两组间无明显差异(P>0.05)。结论局部应用h APN基因重组腺病毒对大鼠骨缺损修复具有促进作用。
Objective To investigate the effect of local administration of recombinant adenovirus of human adiponectin（h APN） Ad-h APN-EGFP on tibial defect repair of SD rats. Methods Tibial defect（2 mm） models of 36 SD rats（72 sides） were randomly divided into three groups（A, B, and C; n=24）. The three groups were injected with Ad-h APN-EGFP, AdEGFP, and normal saline, respectively, during operation and the day after operation. A week after the operation, the expression of h APN and osteogenesis-related factors were detected by real-time polymerase chain reaction. Three weeks after operation, the tibias were examined by micro-computed tomography, hematoxylin-eosin staining, and Masson staining to evaluate the restoration of bone defects. Results 1） The h APN expression was detected in group A but not in groups B and C. Osteogenesis-related factors expression of group A was significantly higher than that of the other groups（P0.05）. 2） Osteogenesis（including bone mineral density, relative bone volume, trabecular number and trabecular thickness） in group A were more evident than those in groups B and C（P0.05）. No significant differences were found between groups B and C（P0.05）. Conclusion Local administration of recombinant adenovirus Ad-h APN-EGFP may be an effective strategy to improve the restoration of bone defects in vivo.
出处
《华西口腔医学杂志》
CAS
CSCD
北大核心
2015年第4期361-365,共5页
West China Journal of Stomatology
基金
四川省科技厅科技支撑计划基金资助项目(2014SZ0019-4)
四川大学优秀青年学者基金资助项目(2012SCU4A12)
关键词
脂联素
腺病毒
骨缺损
成骨
adiponectin
adenovirus
bone defect
osteogenesis
