摘要
该文对OPG基因敲除小鼠进行micro-CT扫描,进行全身骨骼三维重建,并分析股骨结构。结果发现OPG基因敲除纯合子小鼠全身骨骼出现弥漫性蜂窝状低密度影。OPG基因敲除小鼠的骨密度、骨结构模型指数、骨小梁厚度、骨体积分数、骨表面积与体积比、连接密度以及皮质骨厚度等指标均明显小于同窝野生型小鼠,而两基因型小鼠在骨小梁数目、骨小梁分离度及各项异性度等方面无明显差异。以上数据结果表明,OPG基因敲除导致小鼠出现骨质疏松。应用micro-CT可以对小鼠骨进行无创微结构分析,较体视学方法具有一定的优势。
OPG knockout mice were scanned by micro-CT. The reconstruction of the three-dimension mouse skeleton showed the widespread honeycomb shaped low density areas in OPG knockout mice. And we found that OPG knockout mice with lower level ofBMD, SMI, Calibration oftrabecular thickness, BV/TV, BS/BV,connectivity density and cortical bone thickness in comparison to wild-type littermates. But there was no any significant differences in the trabecular number, trabecular separation or DOA in both genotypes. The deletion of OPG in mice leads to osteoporosis. Micro-CT can be used to study bone architecture noninvasively in mice with some advantage over stereological measures.
出处
《中国细胞生物学学报》
CAS
CSCD
北大核心
2012年第1期11-17,共7页
Chinese Journal of Cell Biology
基金
国家高技术研究发展计划(No.2008AA02Z126)
上海市科委项目(No.10140901400)资助项目~~