摘要
以水解度为指标,优化了中性蛋白酶酶解鸡胸软骨Ⅱ型胶原的条件,在此基础上制备不同水解度Ⅱ型胶原酶解复合物,并以淋巴细胞增殖率为指标对其免疫活性进行研究。结果表明,中性蛋白酶的酶解能力最强,其最佳酶解条件为：酶解温度50℃、p H 7.5、底物质量浓度25 mg/m L、加酶量40 U/mg、酶解时间150 min。当Ⅱ型胶原在水解度为18%时,其对淋巴细胞的增殖活性达到58.69%。Ⅱ型胶原酶解复合物的质量浓度对淋巴细胞的增殖率也有显著影响,当其质量浓度达到1 mg/m L时,对淋巴细胞增殖能力达到最大。免疫活性较高的Ⅱ型胶原酶解复合物的分子质量主要分布于1 000~180 D范围内,占其总含量的75.21%。
The hydrolysis conditions of type II collagen from chicken sternal cartilage were optimized to obtain increased degree of hydrolysis（DH）.Based on the optimal hydrolysis parameters,enzymatic hydrolysates with different DHs were prepared,and then lymphocyte proliferation test was utilized to evaluate their immunological activity.The results showed that neutral protease was the most suitable enzyme for hydrolyzing type II collagen and the optimal hydrolysis conditions were hydrolysis temperature of 50 °C,hydrolysis pH of 7.5,collagen concentration of 25 mg/mL,enzyme dosage of 40 U/mg,and hydrolysis time of 150 min.The hydrolysates with a DH of 18%could result in proliferation of lymphocytes by up to58.69%,and the maximal proliferative activity of lymphocytes was found at a concentration of 1 mg/mL.The molecular weight distribution of collagen hydrolysates with higher immunological activity was 1 000-180 D（accounting for 75.21%of the total hydrolysates）.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2015年第13期84-88,共5页
Food Science
基金
国家高技术研究发展计划(863计划)项目(2013AA102207)
国家自然科学基金青年科学基金项目(31201421)
关键词
Ⅱ型胶原
免疫活性肽
中性蛋白酶
淋巴细胞增殖活性
type II collagen
immunologically active peptide
neutral protease
lymphocyte proliferation
