摘要
目的 探讨类风湿关节炎(RA)患者Ⅱ型胶原 (CⅡ)特异性T细胞增殖和CⅡ抗体生成,及与人类白细胞抗原(HLA)- DR_4亚型之间的关系。方法 将CⅡ263 -272十肽与 62例RA患者外周血单个核细胞(PBMC)共孵育 5d,应用四甲基偶氮唑盐法测定T细胞增殖。酶联免疫吸附试验(ELISA)法测定患者血清CⅡ抗体水平。提取 40例患者外周血基因组DNA,以 33种HLA- DR_4等位基因亚型的特异引物PCR扩增后测定患者HLA DR分型情况。结果 在 62例RA患者中, 38例(61 .3% )患者的T细胞在加入CⅡ263- 272后出现明显增殖,CⅡ263 -272抗体阳性率为 53. 2%。细胞增殖反应阳性的患者CⅡ263- 272抗体的阳性率为 66. 7%,增殖反应阴性的患者为 34 6%,两者比较P<0 .05。CⅡ263 -272抗体阳性的患者T细胞增殖反应率为 69 7%,阴性患者为 42 .1%, 两者比较P<0 .05。所有患者T细胞增殖刺激指数 (SI)与CⅡ263- 272抗体浓度呈显著正相关 (r=0 68,P<0. 01)。40例患者HLA分型中,有 16例携带HLA -DR_4等位基因,未发现HLA DR_4与CⅡ特异性T细胞增殖反应、CⅡ抗体阳性有显著相关性。结论 CⅡ抗体的生成可能与CⅡ特异性T细胞介导的B细胞活化有关。
Objective To investigate the proliferation of type Ⅱ collagen specific T cell response and antibody formation in rheumatoid arthritis (RA) and their relations to HLA DR4 subtype. Methods Peripheral blood mononuclear cells and serum were obtained from 62 RA patients, 14 males and 48 females, aged 43±29. CⅡ263 272 decapeptide and the peripheral blood mononuclear cells (PBMCs) from 62 RA patients were co incubated for 5~7 days. MTT method was used to examine the T cell proliferation. The serum antibodies specific to CⅡ 263 272 were examined by ELISA. HLA DR typing was detected in 40 patients by SSP PCR. Results Positive T cell response challenged by CⅡ 263 272 decapeptide was observed in 38 (61 3%) RA patients. The positive rate of CⅡ 263 272 antibody was 66 7% in the T cell response positive group, significantly higher than that of the T cell response negative group (34 6%, P <0 05). The T cell response rate of the CⅡ 263 272 antibody positive group was 69 7%, significant higher than that of the negative group (42 1%, P <0 05). In all patients, the stimulation index (SI) of T cell proliferation to CⅡ263 272 peptide was positively correlated with the level of antibody ( r =0 68, P <0 01). HLA DR4 allele was detected in 16 out of the 40 patients. Positive T cell response and presence of antibody to CⅡ 263 272 were not associated with HLA DR4 phenotype. Conclusion The formation of CⅡ antibody may be related to B cell activation mediated by CⅡ specific T cell.
出处
《中华医学杂志》
CAS
CSCD
北大核心
2005年第6期370-373,共4页
National Medical Journal of China
基金
国家杰出青年基金资助项目 ( 30025040 )
北京市自然科学基金资助项目(7002023)
