摘要
目的:建立地龙的高效液相色谱(HPLC)特征图谱,比较广地龙与沪地龙的主要成分含量差异以及地龙与3种其他动物药的图谱差异。方法:采用地龙HPLC特征图谱法,测定了16份广地龙、8份沪地龙、3份土鳖虫、3份水蛭与3份蜣螂虫的特征图谱,采用相似度评价与t检验对这5种动物药的图谱数据进行差异分析。结果:本研究建立了地龙药材的HPLC特征图谱法,首次指认了11个共有特征峰,包括6个核苷、4个核碱基与1个氨基酸;建立的HPLC特征图谱法可以将地龙与土鳖虫、水蛭及蜣螂虫区分开来;首次对广地龙与沪地龙HPLC特征图谱中主要成分的含量差异进行了研究,其中广地龙中黄嘌呤及腺苷含量高于沪地龙,沪地龙中尿苷、鸟苷及2′-脱氧鸟苷含量高于广地龙,并据此计算出5种成分的峰面积比值新指标S,S值可用于区分2种地龙。结论:该特征图谱可用于地龙药材的鉴别,且可为广地龙与沪地龙的药效学差异研究提供参考。
OBJECTIVE：To establish the HPLC characteristic chromatogram of pheretima,and compare the differences of the main ingredient contents of Guangdong pheretima and Shanghai pheretima and the chromatogram differences among pheretima and3 other animal drugs. METHODS：Pheretima HPLC characteristic chromatogram method was adopted to determine the characteristic chromatograms of 16 Guangdong pheretima,8 Shanghai pheretima,3 eupolyphaga,3 hirudo and 3 catharsius. Similarity evaluation and t test were used to analyze the differences of chromatogram data of 5 animal drugs. RESULTS：The established HPLC characteristic chromatogram method firstly identified 11 common characteristic peaks,including 6 nucleosides,4 nucleobase and 1 amino acid;and it could be used for the identification of pheretima from eupolyphaga,hirudo and catharsius;the differences of main ingredient contents in the characteristic chromatogram of Guangdong pheretima and Shanghai pheretima were firstly studied. The contents of xanthine and adenosine in Guangdong pheretima were higher than Shanghai pheretima,while the contents of uridine,guanosine and 2′-deoxy guanosine in Shanghai pheretima were higher than Guangdong pheretima. A new index S,calculated by these 5 constituents,was successfully applied to distinguish the 2 kinds of pheretima. CONCLUSIONS：The characteristic chromatogram can be used for the identification of pheretima,and can provide reference for the pharmacodynamic differences study of Guangdong pheretima and Shanghai pheretima.
出处
《中国药房》
CAS
北大核心
2015年第21期2971-2974,共4页
China Pharmacy
基金
国家科技重大专项课题--重大新药创制(No.2009ZX09502-023)
关键词
地龙
广地龙
沪地龙
高效液相色谱法
特征图谱
Pheretima
Guangdong pheretima
Shanghai pheretima
HPLC
Characteristic chromatogram
