摘要
目的 研究依那普利对2型糖尿病大鼠血浆AngII和AT1受体表达的影响。方法用放射免疫法测定 血浆AngII水平,免疫组化法观察血管和肾脏AT1受体表达。结果 糖尿病大鼠血浆AngII明显高于对照组,应用依那普利后大鼠血浆AngII明显降低。免疫组化染色发现糖尿病大鼠血管内皮细胞、平滑肌细胞和肾脏AT1受体表达明显增加,依那普利治疗组大鼠血管内皮细胞、平滑肌细胞和肾脏AT1受体表达与正常组接近。结论 糖尿病大 鼠血浆AngII升高,血管和肾脏AT1受体表达增加,依那普利可降低糖尿病大鼠血浆AngII水平,下调血管和肾脏 AT受体表达。
Aim To study the plasma angiotensin II (Ang II) levels and the expressions of angiotensin II 1 receptor (AT 1 ) in blood vessels and kidneys in diabetic and high fat diet rats, and the effec ts of enalapril on plasma Ang II levels and the expressions of AT 1 in bloo d vessels and kidneys in diabetic rats. Methods The plasma Ang II level was assayed with 125I-An g II radioimmunoassay, and the expression of AT 1 in blood vessel and kidne y was analyzed with immunohistochemical technique. Results The plasma Ang II level was significantly higher in type 2 diabetic rats (241±49) pg·mL -1 than that in the control (71±22) pg·mL -1, high fat diet group (151±29) pg·mL -1 ( P<0 .01), and enalapril-treated groups (136±25) pg·mL -1 ( P<0. 05). The plasma Ang II levels in high fat diet and in enalapril-treated group s were also significantly higher than that in control group ( P<0.01). W ith immunohistochemical technique, it was found that the expression of AT 1 in endothelial cells of blood vessels, vascular smooth muscle cells, and kidney s in diabetic group increased. The expression of AT 1 in endothelial cells of blood vessels, vascular smooth muscle cells, and kidney in enalapril-treated group was similar to that in control group. Conclusion The plasma Ang II levels and the expression of AT 1 in type 2 diabetic and high fat diet rats increased. Enalapril was shown t o decrease the plasma Ang II level and downregulate the expression of AT 1 in blood vessels and kidneys in type 2 diabetic rats.
出处
《药学学报》
CAS
CSCD
北大核心
2005年第3期208-212,共5页
Acta Pharmaceutica Sinica
基金
国家自然科学基金资助项目(30070881)
南京医科大学校基金资助项目(NY01 50).
