摘要
目的研究自然发酵时间对海带渣中岩藻黄质提取率的影响以及岩藻黄质的纯化工艺。方法在室温、避光条件下,对海带渣进行自然发酵,考察发酵时间对岩藻黄质提取率的影响。并利用大孔树脂（AB-8）对岩藻黄质进行纯化,分别采用不同纯度的乙醇等度洗脱和梯度洗脱,再用硅胶（100-200目）进一步纯化,用不同比例的石油醚和乙酸乙酯进行等度洗脱和梯度洗脱,确定最佳洗脱方式。采用HPLC法测定岩藻黄质含量,Welch Ultimate XB-C18色谱柱（4.6 mm×250 mm,5μm）,流动相为乙腈-水（90%-100%、100%-100%、100%-90%）,检测波长为449 nm,流速为1.0 mL·min^-1,进样量20μL。结果在室温、避光条件下,自然发酵15 d后,海带渣中岩藻黄质提取率最高,为8.3 mg·g^-1,较发酵前提高1.41倍。用AB-8大孔树脂为色谱柱填充材料,确定了以50%、75%、90%乙醇梯度洗脱,岩藻黄质纯度为23.3%,再用硅胶为色谱柱填充材料,进一步纯化,确定了以石油醚-乙酸乙酯（8：2、15：5、7：3）梯度洗脱,最终岩藻黄质纯度达到70.0%。结论本实验研究了自然发酵时间对海带渣中岩藻黄质提取率的影响以及岩藻黄质的纯化工艺,为获得更高纯度的岩藻黄质提供参考。
Objective To determine the effect of natural fermentation time on the extraction rate and purification technology of fucoxanthin in kelp residue. Methods We explored the effect of natural fermentation days on the extraction rate of fucoxanthin by avoiding light at room temperature. With ethanol as the mobile phase, Macroporous resin（AB-8） was used to purify fucoxanthin through degree elution and gradient elution. Then with different proportions of petroleum ether and ethylacetate as the mobile phase, silica gel was used to further purify fucoxanthin through degree elution and gradient elution, respectively. HPLC method was used with ultimate chromatographic column（4.6 mm×250 mm, 5 μm）, acetonitrile-ultra pure water（90%- 100%, 100%- 100%, 100%- 90%） as the mobile phase, the detective wavelength was 449 nm, the flow rate was 1.0 m L·min^- 1, and the injection volume was 20 μL. Results When avoiding light and at room temperature, the extraction yield after 15 d of natural fermentation was 8.3 mg·g^- 1, which was the highest and increased by 1.41 times than without fermentation.gradient elution chromatography was applied to separate and purify fucoxanthin. AB-8 macroporous resin was first used as the column, and 50%, 75% and 90% ethanol were used as the mobile phase. Then we used silica gel column to further separate and purify fucoxanthin and the mobile phase was petroleum ether and ethyl acetate（8 ：2; 15 ：5; 7 ：3）. The purity of fucoxanthin determined by HPLC was 23.3% and 70.0% respectively. Conclusion Natural fermentation time may affect the extraction rate and purification of fucoxanthin in kelp residue, which helps provide a basis for better purification of fucoxanthin.
出处
《中南药学》
CAS
2015年第6期602-605,共4页
Central South Pharmacy
基金
山东省高等学校科技计划项目(No.J14LK61)
济南市科技发展计划资助(No.201303055)
关键词
岩藻黄质
海带渣
自然发酵
色谱柱
纯化工艺
fucoxanthin
kelp residue
natural fermentation
column chromatography
purification
