摘要
目的观察桂枝汤桂芍不同比例配伍对糖尿病心脏自主神经病变(diabetes cardiac autonomic neuropathy,DCAN)的预防作用。方法 60只雄性大鼠,随机分为空白对照组(简称空白组)、模型对照组(简称模型组)、弥可保组和桂枝汤桂芍1:1组(简称桂芍1:1组)、桂枝汤桂芍2:1组(简称桂芍2:1组)、桂枝汤桂芍1:2组(简称桂芍1:2组),每只10只。灌胃1周后,链脲佐菌素(STZ)腹腔注射诱导建立1型糖尿病模型,成模后继续灌胃4周。检测标本中酪氨酸羟化酶(tyrosine hydroxylase,TH)、乙酰胆碱酶转移酶(choline acetyltransferase,CHAT)和神经生长因子(nerve growth factor,NGF)、睫状生长因子(ciliary neurotrophic factor,CNTF)的变化。结果造模4周后,STZ大鼠体重明显低于空白组大鼠,而血糖高于空白组大鼠。模型组、弥可保组及桂芍2:1、1:2、1:1组大鼠体重和血糖组间比较,差异均无统计学意义(P>0.05)。与空白组比较,模型组左心室及室间隔中TH、TH/CHAT、NGF升高,CHAT、CNTF降低,差异均有统计学意义(P<0.05,P<0.01);与模型组比较,弥可保组左心室TH、TH/CHAT降低(P<0.05,P<0.01),CNTF升高(P<0.05),左心室及室间隔CHAT升高,差异均有统计学意义(P<0.05,P<0.01);桂芍1:1组左心室及室间隔中TH、TH/CHAT降低,CNTF升高(P<0.05,P<0.01),CHAT升高(P<0.01),室间隔中NGF降低(P<0.01);桂芍1:2组左心室TH/CHAT降低(P<0.01),左心室及室间隔CHAT、CNTF均升高(P<0.05,P<0.01);与弥可保组比较,桂芍2:1组左心室CHAT降低,TH、TH/CHAT升高,差异均有统计学意义(P<0.05,P<0.01);桂芍1:1组室间隔TH、TH/CHAT降低(P<0.05),左心室及室间隔CHAT、CNTF升高(P<0.05,P<0.01);桂芍1:1组与桂芍1:2组及桂芍2:1组比较,左心室CHAT升高、TH/CHAT降低及室间隔中TH、TH/CHAT降低,CHAT升高;左心室与室间隔中CNTF亦升高,差异均有统计学意义(P<0.01)。结论 STZ模型大鼠出现自主神经损伤,表现出迷走神经活性降低、交感神经活性亢进。桂枝汤可抑
Objective To observe the preventive effect different compatibilities of Ramulus Cinnamomi（RC） and peony in Guizhi Decoction（GD） on diabetic cardiac autonomic neuropathy（DCAN）.Methods Totally 60 male rats were randomly divided into 5 groups,i.e.,the blank control DM group,the model group,the methycobal group,the 1：1（RC/peony） Guishao group,the 2：1 Guishao group,and the 1：2Guishao group,10 in each group.Rats were pretreated with corresponding drugs for 1 week,and then induced diabetes by intraperitoneal injection of STZ.Drugs were administrated by gastrogavage for 4 more weeks after STZ-injection.Enzyme-linked immunosorbent assay（ELISA） was employed to detect levels of tyrosine hydroxylase（TH）,choline acetyltransferase（CHAT）,nerve growth factor（NGF）,and ciliary neurotrophic factor（CNTF） in myocardial homogenates.Results After 4-week modeling,body weight（BW） was obviously lower,but blood glucose（BG） was higher in STZ rats than in rats of the blank control DM group.There was no statistical difference in BW or BG among the 5 groups（P 〉0.05）.Compared with the blank control group,TH,TH/CHAT,and NGF in left ventricle and ventricular septum increased,CHAT and CNTF increased in the model group（P 〈0.05,P 〈0.01）.Compared with the model group,TH and TH/CHAT in left ventricle decreased（P 〈0.05,P 〈0.01）,CNTF in left ventricle increased（P 〈0.05）,CHAT in left ventricle and ventricular septum increased（P 〈0.05,P 〈0.01） in the methycobal group.TH and TH/CHAT in left ventricle and ventricular septum decreased,CNTF in left ventricle and ventricular septum increased（P 〈0.05,P 〈0.01）,CHAT in left ventricle and ventricular septum increased（P 〈0.01）,NGF in ventricular septum decreased（P 〈0.01） in the 1：1 Guishao group.TH/CHAT in left ventricle decreased（P 〈0.01）,CHAT and CNTF in left ventricle and ventricular septum increased（P 〈0.05,P 〈0.01） in the 1：2 Guishao group.Compared with the methycobal group,CHAT in left v
出处
《中国中西医结合杂志》
CAS
CSCD
北大核心
2015年第6期741-745,共5页
Chinese Journal of Integrated Traditional and Western Medicine
基金
国家自然科学基金资助项目(No.81072962)
关键词
桂枝汤
糖尿病
心脏自主神经病变
Guizhi Decoction
diabetes
cardiac autonomic neuropathy
