摘要
目的建立测定乌术便通颗粒中大黄素的含量方法的。方法采用高效液相色谱法,色谱条件为:色谱柱:ZORBAX 300Extend-C18(4.6mm×250mm,4.6μm);流动相:乙腈-水-冰醋酸(60:40:1);检测波长:437nm;柱温:25℃;流量:0.6m L/min。结果大黄素的回归方程为:Y=4624659.974X-6104.3507,r=0.9998(n=6)。大黄素在0.03-0.18μg含量范围内有良好的线性关系,平均加样回收率为96.86%,RSD为1.84%。结论实验证明本方法准确,重复性好,可用于乌术便通颗粒中大黄素的测定。
Objective To establish a method for the determination of emodin in Wushubiantong pills .Methods The HPLC system consisting the ZORBAX 300Extend -C18 column（4.6mm ×250mm,5μm）; the mobile phase： Acetonitrile -water -Acetic Acid（60：40：1）;the UV detector was set at 437nm; the column temperature was 25℃; the flow rate was 0.6mL/min.Results Emodin regression equation：Y =4624659.974 X –6104.3507, r =0.9998（n =6）.The content of emodin in 0.03 -0.18μg range showed good linear relationship , the average recovery of emodin was 96.86%, RSD was 1.84%.Conclusion The assay demonstrated that the method was accurate and repro -ducible, it can be applied in determination of emodin in Wushubiantong pills .
出处
《湖北中医药大学学报》
2015年第3期46-48,共3页
Journal of Hubei University of Chinese Medicine
