摘要
目的探讨患者外周血淋巴细胞亚群的检测对于脓毒血症患者的临床意义,以判断患者的预后。方法回顾性分析2011年11月-2013年11月重症监护病房脓毒血症住院患者共62例,依据患者的预后将其分为死亡组22例、好转组40例,另外选取60名健康人群作为健康对照组;所有受试人员于清晨空腹采集静脉血2ml送检,经过流式细胞仪检测淋巴细胞亚群,分析其与脓毒血症患者预后的相关性,进行准确的预后判断。结果脓毒血症患者CD3+、CD3+CD4+、CD19+、CD16+CD56+比例以及CD4+/CD8+比值明显低于健康对照组,淋巴细胞比例也显著高于健康对照组;而脓毒血症死亡组患者的CD3+、CD3+CD4+、CD19+、CD16+CD56+比例及CD4+/CD8+比值明显低于好转组患者,差异均有统计学意义(P<0.05)。结论脓毒血症患者检测淋巴细胞亚群的比例可判断其预后,预测其死亡的准确度,其中CD3+和CD4+对于预测死亡预后的准确度较高。
OBJECTIVE To discuss the clinical significance of detection of peripheral blood lymphocyte subsets for patients with sepsis so as to improve prognosis.METHODS Totally 62 cases of sepsis patients in ICU from Nov.2011 to Nov.2013 were analyzed retrospectively.They were divided into a dead group with 22 cases and improved group with 40 cases according to the prognosis of patients.Another 60 healthy people were chosen as control group.All subjects received blood sampling by 2 ml with an empty belly in the morning.Lymphocyte subsets were detected by flow cytometry and its correlation with prognosis of sepsis patients were analyzed.RESULTS Proportion of CD3＋,CD3＋CD4＋,CD19＋and ratio of CD4＋/CD8＋in the sepsis groups were significantly lower than the control group and the number in the dead group was significantly lower than the improved group（P〈0.05）.CONCLUSION The proportion of peripheral blood lymphocytes subsets can be used to make a judgment of prognosis and improve the accuracy of death prediction,especially CD3＋and CD4＋.
出处
《中华医院感染学杂志》
CAS
CSCD
北大核心
2015年第14期3125-3126,3129,共3页
Chinese Journal of Nosocomiology
基金
天津市科学基金资助项目(12JCZDJC23498)
