摘要
本实验建立了茵山莲软胶囊中栀子苷含量的测定方法。采用反相高效液相色谱法,选择迪马C18色谱柱(4.6mm×250mm,5μm),以乙腈-水(V乙腈∶V水=13∶87)为流动相,检测波长238nm,流速1.0m L/min,柱温25℃,进样量10μL。栀子苷在0.008 04mg/m L^0.0804mg/m L范围内质量浓度与峰面积呈良好的线性关系,R2=0.9998,平均回收率97.667%,RSD0.567%(n=6)。该试验的测定方法简单准确,且分离效果好,灵敏度较高,可作为茵山莲软胶囊的质量控制方法。
The present study developed a method to determine the levels of geniposide in Yinshanlian soft capsules with RP-HPLC .Geniposide was performed on a Diamonsil C18 column （4.6mm × 250mm ,5μm ） with the mobile phase of acetonit water （V∶V=13∶87 ） .The detection wavelength was set at 238 nm and the column temperature was 25℃ .There had a good linear relationship between geniposide contents and peak area when its concentration localized in the range from 0.008 04mg/mL to 0.0804mg/mL .The recovery rate of geniposide was 97.667% ,RSD were 0.567% .The method we used has the characteristics of simplicity ,accuracy ,as well as high efficency and sensitivity .Thus it can be used to test the quality of Yinshanlian soft capsules .
出处
《特产研究》
2015年第2期45-48,共4页
Special Wild Economic Animal and Plant Research
