摘要
目的改进蓝芩口服液中栀子苷含量的测定方法。方法采用高效液相色谱法测定。色谱柱为Agela MP C18(4.6mm×250mm,5μm),流动相改进为0.05mol/L磷酸氢二钠溶液(pH值6.8)-甲醇(70∶30),流速为1.0ml/min,检测波长为238nm。结果栀子苷进样量在0.169-0.844μg/ml范围内与峰面积呈良好的线性关系(r=1.0000),平均加样回收率为100.28%,相对标准偏差(RSD)为0.34%。结论测定方法改进后,柱效提高,结果准确。
Objective To modify high performance liquid chromatography（HPLC） method for determining the content of geniposide in Lanqin oral solutiora. Methods The geniposide in Lanqin oral solution was determined by HPLC. Chromatographic column was Agela MP Cls （4. 6 minx 250 ram, 5 tim）, Mobile phase was modified as 0. 05 mol/L disodium hydrogen phosphate solution（with a pH value of 6.8） and methanol（70 ： 30）. The flow rate was 1.0 ml/min and the wavelength was 238 nm. Results The linearity was good（r=0. 9999） within the range of 0. 169-0. 844 μg/ml. The average recovery rate was 100, 28% and RSD was 0. 34% （n = 6）. Conclusion The modified method may improve the column efficiency and accuracy of determination. [
出处
《江苏医药》
CAS
北大核心
2013年第23期2817-2819,共3页
Jiangsu Medical Journal
关键词
蓝芩口服液
栀子苷
高效液相色谱法
Lanqin oral solution
Geniposide
High performance liquid chromatography
