摘要
以锦灯笼宿萼为试材,采用动态吸附及解吸的方法,研究各因素对AB-8型大孔树脂富集锦灯笼宿萼总黄酮的影响,明确AB-8型大孔树脂富集锦灯笼宿萼总黄酮的最佳工艺条件。结果表明:上样量为树脂与药材之比1.4∶1,锦灯笼宿萼提取液上样浓度为0.395mg/mL,上样液pH为2.0~3.0,吸附速率为3BV/h,静置15min,吸附后先用2BV蒸馏水洗脱,再用5BV的90%乙醇以1~2BV/h的流速解吸,在此工艺条件下,其平均吸附率为84.41%,平均解吸率为96.22%,富集后锦灯笼宿萼干浸膏中总黄酮含量由原来的6.95%提高到23.62%,树脂富集倍数为3.4倍。
Taking dynamic absorption-desorption as experiments, the effect of many factors on the enrichment of total flavonoids （TF） in persistent calyx of Physalis alkekengi L. var. Franchetii（Mast. ）Makino with AB-8 type macroporous resin were studied. The results showed that the optimum enrichment technology, the loading quantity of sample（resin weight/crude drugs weight）, the concentration and the pH value of the sample, adsorption rate, static time, volume of water,ethanol content,desorption rate, the volume of eluant were 1. 4 ： 1,0. 395 mg/mL, 2. 0-- 3. 0,3 BV/h, 15 rain, 2 BV,90% ,1--2 BV/h and 5 BV respectively, the average absorption and desorption rate were 84. 41% and 96. 22%. The purity of TF in extracts could be changed from 6. 95% to 23. 62% ,which offered about 3. 4 times enrichment with this type of resins.
出处
《北方园艺》
CAS
北大核心
2015年第12期131-135,共5页
Northern Horticulture
基金
吉林省教育厅计划资助项目(吉教科合字[2014]第355号)
关键词
锦灯笼宿萼
AB-8型树脂
总黄酮
富集
persistent calyx of Physalis alkekengi L. var. Franchetii（Mast. ）Makino^AB-8 macroporous absorption resin^total flavonoids ~enrichment
