摘要
目的：运用高效液相色谱-高分辨飞行时间质谱（HPLC-TOF/MS）联用技术快速鉴别肝力保胶囊中化学成分。方法：色谱分离采用SHISEIDO MG C18色谱柱（3.0 mm×100 mm,3μm）;流动相0.1%甲酸水（A）-乙腈（B）,梯度洗脱（0~10min,5%~20%B;10~30 min,20%~70%B）,柱温25℃,流速0.6 m L·min-1,柱后分流比2∶1。质谱定性采用飞行时间质谱,电喷雾离子源（ESI）,正负离子模式扫描（m/z 100~1 000）。结果：一次性鉴别出肝力保胶囊中30种化学成分。结论：该研究比较全面地阐明了肝力保胶囊的化学组成,为该复方的药效学物质基础研究和质量控制奠定了基础。
Objective： To identify chemical composition in Ganlibao capsules by high-performance liquid chromatography-time of flight mass spectrometry（ HPLC-TOF / MS） method. Method： The separation was performed on SHISEIDO MG C18 reverse phase column（ 3. 0 mm × 100 mm,3 μm）. The mobile phase consisted of water containing 0. 1% formic acid（ A）-acetonitrile（ B） was used for gradient eluting（ 0-10 min,5%-20% B; 10-30 min,20%-70% B）. Temperature of column was 25 ℃,the flow rate was 0. 6 m L·min- 1and postcolumn split ratio was 2∶ 1. Time-of-flight mass spectrometer（ TOF / MS） and electrospray ion source（ ESI） were applied for qualitative analysis under positive and negative ion mode,and mass scan range was m / z 100-1 000.Result： The 30 components of Ganlibao capsules were identified by HPLC-TOF / MS. Conclusion： This method is simple and rapid for elucidating the constituents of Ganlibao capsules,which provides the basis for the research of active composition and quality control for this prescription.
出处
《中国实验方剂学杂志》
CAS
CSCD
北大核心
2015年第13期70-73,共4页
Chinese Journal of Experimental Traditional Medical Formulae
