摘要
目的：建立短葶山麦冬皂苷C差向异构体含量测定方法,测定不同采收期短葶山麦冬中短葶山麦冬皂苷C差向异构体含量。方法：采用HPLC-UV法,Waters XBridge C18色谱柱（4.6 mm×250 mm,3.5μm）,流动相乙腈-水（42∶58）,检测波长203 nm,柱温40℃,流速1.0 m L·min-1。结果：25R-短葶山麦冬皂苷C和25S-短葶山麦冬皂苷C分别在20.40~408.00 mg·L-1（R2=0.999 9）,20.09~401.80 mg·L-1（R2=0.999 9）呈良好线性关系,平均回收率分别为99.85%（RSD 2.1%）,101.40%（RSD 1.8%）;8批不同采收期短葶山麦冬中25R-短葶山麦冬皂苷C,25S-短葶山麦冬皂苷C分别为0.081%~0.136%,0.012%~0.150%。结论：该方法简便、快速,可准确测定短葶山麦冬皂苷C差向异构体含量;不同采收期短葶山麦冬中短葶山麦冬皂苷C差向异构体含量差异较大,25S-短葶山麦冬皂苷C含量变化尤为明显,应严格控制短葶山麦冬采收期。
Objective： To establish a method for the determination of C-25 epimers of ruscogeninl-O- [/3-D-glucopyranosyl （1 → 2） ] [β-D-xylopyranosyl （1 →3 ） 1 -β-D-fucopyranoside in the tuberous roots of Liriope muscari at different harvest time. Method： A Waters XBridge Cls column （4.6 mm × 250 mm, 3.5 μm） was used for separation at 40 ℃ with a flow rate of 1.0 mL ·min- 1. The wavelength of detector was set at 203 nm and the mobile phase consisted of acetonitrile-water （42： 58）. Result： The linear ranges of 25R-and 25S-epimer were 20.40-408.00 mg·L-1 （R2 =0.999 9） and 20.09-401.80 mg·L-1 （R2 =0.999 9）. The average recoveries were 99.85% and 101.40% , and RSD were 2.1% and 1.8% , respectively. 8 batches of L. muscari at different harvest time were analyzed by HPLC, the contents of 25R-, 25S-epimer and were O. 081%-0. 136%, 0. 012%- 0. 150%. Conclusion： The method is simple, sensitive and accurate, it can be used for determination of C-25 epimers of ruscogeninl-O- [β-D-glucopyranosyl （1 → 2） ] [β-D-xylopyranosyl （1 →3） ] -β-D-fucopyranoside . The contents of 25R-and 25S-epimer in the tuberous roots of L. muscari at different harvest time were significantly different, especially 25S-epimer, which indicate that the harvest time of L. muscari should be controlled strictly.
出处
《中国实验方剂学杂志》
CAS
CSCD
北大核心
2015年第14期28-31,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家自然科学基金项目(81274004
81473317)
国家"重大新药创制"科技重大专项(2012ZX09102201-015)
关键词
短葶山麦冬
短葶山麦冬皂苷C
差向异构体
含量测定
采收期
Liriope muscari
ruscogeninl-O- [β-D-glucopyranosyl （ 1 → 2 ）] [β-D-xylopyranosyl（ 1 →3 ） ] -β-D-fucopyranoside
epimer
determination
havest time
