摘要
目的考察均相酶扩大免疫分析法(enzyme-multiplied immunoassay test,EMIT)和高效液相色谱法(high performance liquid chromatography,HPLC)分别测定癫痫患儿丙戊酸钠血药浓度的相关性,为临床测定丙戊酸钠(sodium valproate,VPA)血药浓度方法学选择提供依据。方法收集服用丙戊酸钠的癫痫患儿血样200例,分别用均相酶扩大免疫分析法和高效液相色谱法进行测定,对结果的相关性进行分析。结果以HPLC法测定结果(X)与EMIT法测定结果(Y)作回归方程如下:Y=1063.517X-331.351(r=0.933),此2种测定方法具有显著相关性(P<0.05﹚,EMIT法测定血浆VPA浓度较HPLC法偏高。结论均相酶扩大免疫分析法和高效液相色谱法测定癫痫患儿丙戊酸钠血药浓度结果具有显著相关性,临床在进行丙戊酸钠血药浓度测定中应根据实际情况选择方法。
Objective To study the correlation of blood drug concentration determination of sodium valproate in children with epilepsy by using enzyme-multiplied immunoassay test （EMIT） and high performance liquid chromatography （HPLC）, so as to provide basis of selection in clinical determination of sodium valproate （ VPA） .Methods 200 blood samples of epileptic children taking sodium valproate were collected, blood concentration were determined by EMIT and HPLC method, and the correlation was analyzed.Results The following regression equation was obtained by determination results of HPLC （X） and EMIT （Y）： Y=1063.517X-331.351（r=0.933）, which had significant correlation （P〈0.05）.The plasma concentration of VPA determined by EMIT was higher than that by HPLC.Conclusion The determination results of VPA concentration in epileptic children by using EMIT and HPLC has significant correlation, and it should choose the suitable method to determine the blood drug concentration of VPA based on the actual situation.
出处
《中国生化药物杂志》
CAS
2015年第4期169-172,共4页
Chinese Journal of Biochemical Pharmaceutics
关键词
均相酶扩大免疫分析法
高效液相色谱法
丙戊酸钠
血药浓度
enzyme-multiplied immunoassay test
high performance liquid chromatography
sodium valproate
blood drug concentration
