摘要
为确定烟草转录因子Nt WRKY-R1的转录激活域,本研究将Nt WRKY-R1的编码区分为7个不同片段,分别构建到酵母表达载体p GBKT7中与GAL4的DNA结合域融合,通过酵母双杂交试验确定,Nt WRKY-R1本身具有能够激活GAL4 BD转录起始的激活结构域,并将转录激活区定位于该转录因子蛋白肽链的N端75个氨基酸残基和C端80个氨基酸残基中。
To identify the activation domain of tobacco transcription factor NtWRKY-R1,coding region of NtWRKY-R1 was separated to seven fragments and then were fused with GAL4 BD （ DNA binding do-main） on pGBKT7 vector in this work. The yeast two hybridization assay showed that NtWRKY-R1 could activate the transcription initiation of GAL4 BD,and the activation domain of NtWRKY-R1 was localized in 75 residues at N-terminus and 80 residues at C-terminus of NtWRKY-R1 .
出处
《河南农业科学》
CSCD
北大核心
2015年第6期34-37,共4页
Journal of Henan Agricultural Sciences
基金
国家自然科学基金项目(30971704)
