摘要
NAC（NAM，ATAF1/2，CUC2）转录因子是植物所特有的转录因子家族，其参与植物生长发育、激素调控和胁迫响应等众多生理生化过程。对16个玉米NAC转录因子进行了序列特征、基因结构和保守区域分析发现，它们均含有保守的NAC结构域和不稳定的转录激活域，编码的氨基酸长度变异幅度为174～1467aa。通过qRT-PCR发现，水分胁迫下NAC基因在玉米幼苗根茎叶中均有不同程度的上调或下调，其中，GR—MZM2G014653_P01和GRMZM2G180328_P01这2个基因在水分胁迫条件下，不同胁迫时期在根茎叶中都呈现上调表达，各时期上调表达的总量达到对照的221倍。试验证明，部分NAC转录因子参与了玉米对干旱胁迫的响应过程，在玉米耐旱遗传改良中具有潜在的实际应用价值。
NAC （NAM, ATAF 1/2, CUC2） is one plant-specific transcription factor family and NAC proteins participate in a variety of plant physiological and biochemical processes, such as the developmental process, hormone regulation and stress responses. In this study, 16 maize NAC transcription factors were analyzed by the sequence characteristics, gene structure and conserved regions, and we found that all of NAC proteins contain NAM conserved domain and variable transcriptional regulatory region and the length of their encoded amino acid ranged from 174-1 467 aa. There were different expression patterns of tissues under water stress. Their expression varied up or down in roots, stems and leaves of maize seedlings by qRT-PCR detection. GRMZM2GO14653_PO1 and GRMZM2G180328_PO1 were up-regulation in all three tissues and all stress periods under water-stressed conditions, and the highest expression were involved in stress-response process and had potential application value in the genetic improvement of drought tolerance in maize.
出处
《山西农业科学》
2014年第4期307-312,共6页
Journal of Shanxi Agricultural Sciences
基金
国家自然科学基金项目(31271735)
国家"863"计划项目(2011AA100501)
关键词
玉米
NAC转录因子
水分胁迫
表达模式
maize
NAC transcription factor
water stress
expression pattern
