摘要
目的探讨番茄红素对急性大鼠脊髓损伤后核转录因子表达及对神经功能恢复的作用。方法健康成年SD大鼠36只,采用Allen法(打击强度×打击高度:10 g×25 mm)在T9造成大鼠脊髓损伤模型,并随机分为对照组、甲泼尼龙琥珀酸钠(MP)组和番茄红素组,每组12只。造模成功30 min后,对照组不给予治疗,MP组腹腔注射MP 30 mg/kg,番茄红素组灌胃番茄红素20 mg/kg。于术后1,3,7 d分别对各组进行后肢功能Basso Beattie Bresnahan(BBB)评分。随后处死大鼠取材获损伤段脊髓,切片行免疫组织化学染色检测核转录因子(NF-κB)的表达。结果与对照组相比,术后1,3,7 d番茄红素组BBB评分均明显增高(P<0.05),MP组1 d、7 d评分显著高于对照组(P<0.05)。术后1 d番茄红素组的NF-κB表达明显低于对照组(P<0.001),3 d和7 d MP组和番茄红素组NF-κB的表达均明显低于对照组(P<0.001)。结论番茄红素可以通过抑制NF-κB的表达降低急性脊髓损伤后的炎症反应对组织的损伤,并促进大鼠神经和运动功能恢复。
Objective To explore the effects of lycopene on NF-KB expression and the recovery of neurological function after spinal cord injury in rats. Methods Spinal cord injury model at T9 was made by modified Allen technique（ 10 g ×25 mm）. Thirty-six healthy adult SD rats were randomly divided into control group, methylprednisolone sodium succinate（MP） group and lycopene group （n = 12 in each group）. At 30 min after successful modeling, the rats were not treated in control group, while the rats were injected with MP 30 mg/kg in MP group, and lycopene 20 mg/kg in lycopene group. The recovery of the locomotor function of each group was evaluated with Basso, Beattie and Bresnahan（BBB） scale at 1 d, 3 d, 7 d after injury, and then the rats were kiUed to detect the ex- pression of nuclear transcription factor（NF-KB） by immunohistochemical staining. Results At 1 d, 3 d, 7 d, BBB scores in lyco- pene group were significantly higher than in control group （ P 〈 0.05 ）. The BBB scores at 1 d, 7 d were significantly higher in MP group than in control group（P 〈 0.05）. NF-KB expression was significantly lower in lycopene group than in control group at 1 d （P 〈 0.001 ）, and it was also significantly lower in MP group and lycopene group than in control group at 3 d and 7 d （P 〈 0.001 ）. Con- clusion Lycopene can inhibit expression of NF-KB in acute spinal cord injury to reduce the tissue damage caused by inflammatory re- action and promote rat nerve and motor function recovery.
出处
《山西医科大学学报》
CAS
2015年第6期532-535,608,共5页
Journal of Shanxi Medical University
基金
国家级大学生创新创业训练计划资助项目(201313705002)
关键词
番茄红素
脊髓损伤
核转录因子
炎症
lycopene
spinal cord injury
nuclear transcription factor
inflammation
