摘要
目的观察促红细胞生成素(EPO)对大鼠急性脊髓损伤后脊髓组织核因子κB(NF-κB)表达及肿瘤坏死因子α(TNF-α)、白细胞介素1β(IL-1β)、IL-6水平的影响,探讨其减轻急性脊髓损伤后继发性脊髓损伤的机制。方法 18只SD大鼠随机均分为对照组、损伤组和治疗组,用动脉瘤夹从两侧夹闭脊髓30 s造成脊髓损伤模型。治疗组分别予术后1 h和1、2、3 d腹腔注射EPO 5000U/kg。术后3 d处死大鼠取材,用EMSA法检测脊髓组织中NF-κB活性,ELISA法检测TNF-α、IL-1β、IL-6表达。结果治疗组脊髓组织中NF-κB、TNF-α、IL-1β、IL-6的水平均比损伤组显著降低(P<0.05)。结论 EPO能下调急性脊髓损伤后脊髓组织中炎症因子的表达,从而抑制炎症反应,对继发性脊髓损伤有一定的保护作用。
Objective To observe the influence of erythropoietin（EPO） on the expressions of nuclear factor-kappa B（NF-κB） and inflammatory factors of tumor necrosis factor-α（TNF-α）,interleukin（IL）-1β and IL-6 following acute spinal cord injury and to explore the possible mechanism of reducing secondary spinal cord injury.Methods Eighteen SD rats were randomly and equally divided into 3 groups of sham control group,injury model group and treatment group.Treatment group was given intraperitoneal injection of EPO（5000U/kg） at 1h,on the 1st,2nd and 3rd day after establisgment of spinal cord injury model.All rats were sacrificed after 3 days.The expression of NF-κB was detected by EMSA assay and the expressions of TNF-α,IL-1β and IL-6 were examined with ELISA.Results The levels of NF-κB,TNF-α,IL-1β,IL-6 in spinal cord tissue of treatment group were significantly lower than those in injury model group（P0.05）.Conclusion EPO administration can down-regulate the expressions of the inflammatory factors,inhibit the inflammatory response and play a role in protecting the secondary spinal cord injury.
出处
《江苏医药》
CAS
CSCD
北大核心
2011年第4期381-383,共3页
Jiangsu Medical Journal
