摘要
目的 探讨胰腺脂肪浸润对营养性肥胖大鼠的胰岛功能及形态影响。方法 刚断乳SD雄性大鼠70只,随机分为普通饲料组（N组）和高脂饲料组（F组）,18周后成功构建营养性肥胖大鼠模型34只,将其按体重分为轻度肥胖组（A组,n=11）、中度肥胖组（B组,n=11）、重度肥胖组（C组,n=12）。测空腹胰岛素（FINS）、游离脂肪酸（FFA）、白介素-6（IL-6）、肿瘤坏死因子α（TNFα）、胰腺组织甘油三酯（TG）和胰腺组织病理改变等指标。计算胰岛素抵抗指数（HOMA-IR）、胰岛素β细胞功能指数（HOMA-β）、早期胰岛素分泌功能指数（ΔI30/ΔG30）及胰岛素曲线下面积（0~120 min）。结果 C组大鼠胰腺TG含量比N组明显增高[（324.52±55.82）μmol/L vs（269.57±50.45）μmol/L,P〈0.05）]。与N组相比,B组HOMA-IR显著升高[（2.27±0.31）vs（1.91±0.26）,P〈0.01];B、C组的ΔI30/ΔG30明显降低[（8.90±2.23）vs（12.56±3.38）,P〈0.05;（7.41±2.00）vs（12.56±3.38）,P〈0.01]。结论 轻中度胰腺脂肪浸润可造成胰岛素早期分泌时相延迟,出现胰岛素抵抗;而重度胰腺脂肪浸润可致胰岛β细胞损伤。
Objective To study the morphology and impact of pancreatic fatty infiltration on pancreatic islet in diet- induced obesity rats. Methods Seventy male weanling Sprague-Dawley （SD） rats were randomly divided into two groups：normal chow group（ group N）and high-fat diet group（ group F）. Obesity model covering 34 rats was established successfully after 18 weeks. The obese rats were then divided into three groups according their weight： a mild-obesity group （ group A, n = 11 ）, a moderate-obesity group （ group B, n = 11 ） and a severe-obesity group （ group C, n = 12 ）. Fast- ing plasma insulin, free fatty acid（ FFA）, interleukin 6 （ IL-6 ）, tumor necrosis factor alpha （ TNFa ）, and pancreatic tri- glyceride（TG） were determined and pancreatic pathological changes were observed. In addition, insulin resistance index （homeostatic model assessment for insulin resistance, HOMA-IR）, insulin β cell function index （HOMA-β）, early phase insulin secretion index（ AI30/AG30 ）and area under curve of insulin （0 - 120 minutes）were evaluated. Results Compared with group N,pancreatic TG was significantly higher in group C（324. 52 ± 55. 82 p.mol/L vs. 269. 57 ± 50. 45 txmol/L, P 〈0. 05） ;HOMA-IR was significantly higher in group B（2. 27 ±0. 31 vs. 1.91 ±0. 26,P 〈0. 01 ） ;I30/AG30 was sig- nificantly lower in group B and C（8.90 ±2.23 vs. 12. 56 ±3.38,P 〈0.05 and 7.41 ±2.00 vs. 12.56 ±3.38, P 〈0. 01 ）. The area under the curve of insulin （0 -120 minutes）was significantly increased in group B （5867.41 ± 476. 27 vs. 4976. 90 ± 654. 93, P 〈 0. 01 ）. Conclusion Mild-moderate pancreatic fatty filtration could lead to the delay of insulin secretion in early phase and insulin resistance, while the severe pancreatic fatty filtration could cause the dam- age of islet β-cells.
出处
《中国公共卫生》
CAS
CSCD
北大核心
2015年第6期784-787,共4页
Chinese Journal of Public Health
基金
山东省科技发展计划项目(2013GSF11860)
