摘要
以5个葡萄柚品种的无菌苗带腋芽茎段为材料,研究丛生芽的诱导、增殖及生根培养等,旨在构建葡萄柚的离体快繁体系。结果表明,在6-BA浓度为0.25和0.50 mg·L^-1时,腋芽萌发率可达100.00%;0.10-0.40 mg·L^-1的IBA处理利于腋芽的诱导和生长。在MS＋0.50 mg·L^-16-BA＋0.20 mg·L^-1IBA条件下,火焰的萌芽率最高,为88.89%。MS＋0.50 mg·L^-16-BA＋0.20 mg·L^-1IBA培养基适宜火焰、矮化、瑞路比、里约红丛生芽的增殖。在MS＋0.25 mg·L^-16-BA＋0.20 mg·L^-1IBA培养基条件下,星路比增殖系数达到最大。1/2 MS＋1.00 mg·L^-1IBA生根培养基适合火焰、瑞路比、里约红、星路比生根。里约红移栽成活率最高,达81.82%;瑞路比最低,仅39.39%。
To construct a rapid in vitro propagation system for grapefruit, the stems with axillary buds of aseptic seedlings from five grapefruit varieties were used as explants to study the multiple shoots induction, proliferation and rooting of grapefruit. The results were as follows ： The highest germination rate was observed in the medium with 0. 25 and 0. 50mg·L^-16-BA, which reached 100.00%. The medium with 0.10 -0.40 mg·L^-1 IBA was optimum medium for inducing and growth of axillary bud. The best medium for germination of Flame was MS ＋0. 50 mg·L^-1 6-BA ＋ 0.20mg·L^-1 IBA, resulting in 88.89% of germination rate, this medium was also suitable for proliferation of the regeneration buds from Flame, Genetic Dwarf, Ray Ruby and Rio Red, and the best proliferation medium for Star Ruby was MS ＋ 0. 25 mg. L 1 6-BA ＋ 0. 20 mg·L^-1 IBA.The best rooting medium for Flame, Ray Ruby, Rio Red and Star Ruby was 1/2 MS ＋ 1.00mg·L^-1 IBA. The highest survival rate of regenerative plant was observed in Rio Red, which reached 81.82% , while the lowest was observed in Ray Ruby, only 39. 39%.
出处
《浙江农业学报》
CSCD
北大核心
2015年第5期776-781,共6页
Acta Agriculturae Zhejiangensis
基金
云南省教育厅重点项目(2014C006Z)
国家林业局948项目(2011-4-45)
关键词
葡萄柚
增殖
腋芽
快速繁殖
Citrus paradisi Macf.
proliferation
axillary bud
rapid propagation
