摘要
以美国红枫腋芽茎段为外植体,采用组织培养方法,研究了不同激素配比对美国红枫无菌体系的建立、启动、增殖、壮苗培养、试管苗生根移栽的影响。结果表明:带腋芽茎段最佳消毒方式为以70%酒精浸泡30s,1%升汞消毒6min,成活率和抽芽率最高,分别达到75.22%和95.13%;NAA对启动培养的影响大于6-BA,接种于培养基MS+NAA 0.05mg·L^(-1)+6-BA0.5mg·L^(-1)中的外植体诱导率最高,为62.80%,培养基及激素的最佳配比为NN69+2,4-D0.1mg·L^(-1)+TDZ 0.3mg·L^(-1),诱导率达到80.21%;增殖培养阶段的最佳培养基为NN69+NAA 0.1mg·L^(-1)+TDZ 0.3mg·L^(-1),增殖系数为4.2;MS+2,4-D 0.1mg·L^(-1)+6-BA0.5mg·L^(-1)能有效促进小苗的生长;生根培养最适培养基MS+NAA 0.1mg·L^(-1)+2,4-D0.5mg·L^(-1),生根率达99.8%,生根条数达7.9,根长达6.5cm;练苗后移栽到蛭石+珍珠岩(1∶1)基质中,移栽成活率为75.2%。
Taking Acer rubrum as material,using transformation technology,the whole process from the sterile system establishment,start up cultivation,breeding,seedling cultivation,root culture and hardening-seedling transplanting were discussed by different homone proportion.The results showed that the best disinfection way of the stem with bud explants was disinfection by 70% alcohol immersion 30seconds＋6minutes disinfection by 1% mercuric chloride.The highest survival rate and sprout rate were 75.22%and 95.13%.Adopting orthogonal test,medium and hormone ratio test to start training.The former result showed that the influence of NAA was greater than 6-BA,MS＋NAA 0.05mg·L-1＋6-BA 0.5mg·L-1 had the highest start rate of 62.80%.The latter result showed NN69＋2,4-D 0.1mg·L-1＋TDZ0.3mg·L-1 had the highest start rate of 80.21%.The best medium for multiplication culture was NN69＋NAA0.1mg·L-1＋TDZ 0.3 mg·L-1,the multiplication factor was 4.2.The MS＋2,4-D 0.1 mg·L-1＋6-BA0.5mg·L-1 could effectively promote the growth of test-tube seedlings.In root culture stage,the optimal culture medium was MS＋NAA 0.1mg·L-1＋2,4-D 0.5mg·L-1.The rooting effect was best with root rate 99.8%,root number 7.9,root length 6.5cm.Amounts of elements benefits test-tube plant rooting.After the culture-bottle seedlings were transplanted into the matrix composed of vermiculite and perlite（1∶1,V∶V）,the transplanting survival rate was up to 75.2%.
出处
《北方园艺》
CAS
北大核心
2016年第20期97-102,共6页
Northern Horticulture
基金
江苏高校优势学科建设工程资助项目(PAPD)
关键词
美国红枫
组织培养
生根
Acer rubrum
tissue culture
rooting
