摘要
目的：研究白细胞介素‐6（IL‐6）、IL‐10和IL‐23在小鼠病毒性心肌炎（VMC）中的表达，为临床治疗VMC提供相应的理论依据。方法将65只4周龄的雄性小鼠随机分成对照组25只、V M C组40只，分别设置0、1、2、4、6周5个时间点，其中VMC每亚组为8只，而对照组的亚组为5只，对VMC组各小鼠在腹腔内注射0．1 ml的病毒液，而对照组则注射0．1 ml的磷酸盐缓冲液（即PBS ），对比各时期两组小鼠心肌组织内IL‐6、IL‐10、IL‐23 mRNA水平及蛋白水平。结果实验组小鼠在感染柯萨奇病毒（CVB）后的2～4周内心肌组织产生的炎症反应最为明显，且 IL‐6、IL‐10及 IL‐23 mRNA 在各时期的表达量均显著高于对照组，差异均有统计学意义（ P＜0．05）；实验组在0～6周各时期中的IL‐6、IL‐10及IL‐23蛋白水平均显著高于对照组相应时期，差异均有统计学意义（P＜0．05）。结论 IL‐6、IL‐10及IL‐23可能与VMC发病过程有关，临床上应加以重视。
OBJECTIVE To investigate IL‐6 ,IL‐10 and IL‐23 expression in murine viral myocarditis （VMC） ,so as to provide theory information for clinical treatment of VMC .METHODS Totally 65 male four‐week‐old mice were randomly divided into the control group （25 cases） ,and VMC group （40 cases） ,in each subgroup of VMC had 8 cases ,while each subgroup of controls had 5 cases .Then five time points （0 week ,one week ,two weeks ,four weeks ,six weeks） were set .Each mouse in the VMC group was injected intraperitoneally with 0 .1 ml of the virus solution ,while the control group injected with 0 .1 ml phosphate buffered solution （PBS） .The mRNA and protein levels of IL‐6 ,IL‐10 and IL‐23 at each time point in the two groups were compared .RESULTS At 2 to 4 weeks after infection of coxsackie virus （CVB） in the experimental group ,the inflammatory reaction in myocardial tissues was the most obvious ,and IL‐6 ,IL‐10 and IL‐23 mRNA expression levels in each period were significantly higher than the control group （P〈 0 .05） .The IL‐6 ,IL‐10 and IL‐23 protein levels in the experimental group were significantly higher than those in the control group at 0 week to six weeks （P〈0 .05） .CONCLUSION IL‐6 ,IL‐10 and IL‐23 may be related to the pathogenesis of VMC ,which clinical attention should be paid to .
出处
《中华医院感染学杂志》
CAS
CSCD
北大核心
2015年第12期2655-2657,共3页
Chinese Journal of Nosocomiology
基金
广东省自然科学博士基金资助项目(10451008901006170)
