摘要
目的 探讨TNF-α、单核细胞趋化蛋白-1（MCP-1）在急性病毒性心肌炎（VMC）发病中的作用及 β受体阻滞剂卡维地洛对其影响。方法 将80只4周龄雄性Balb/c小鼠随机分为3组：心肌炎组（n=30）、卡维地洛组（n=30）和对照组（n =20），心肌炎组及卡维地洛组经腹腔接种柯萨奇病毒B 3（CVB 3）诱发急性 VMC，对照组接种不含病毒的Eagle′s液；卡维地洛组于接种病毒次日开始灌服卡维地洛5 mg/kg，心肌炎组和对照组灌服9 g/L盐水，每天2次。于接种后的第7、14天随机从各组抽取8只小鼠取血后处死，HE染色检测小鼠心肌病理积分，ELISA法检测血清TNF-α水平，RT-PCR检测心肌组织 MCP-1 mRNA 的表达。结果 心肌炎组心肌组织病理积分较对照组明显增高（P 〈0.01），卡维地洛组较心肌炎组病理积分降低（P 〈0.05）；心肌炎组小鼠血清TNF-α水平较对照组升高（P 〈0.01），且与心肌病理积分呈正相关（r=0.42，P 〈0.05），卡维地洛组TNF-α水平较心肌炎组显著下降（P 〈0.05）；与对照组比较，心肌炎组小鼠心肌MCP-1 mRNA表达明显增加（P 〈0.01），卡维地洛组小鼠心肌 MCP-1 mRNA 表达较心肌炎组明显下降（P 〈0.05）。结论 TNF-α 和 MCP-1参与VMC的发病过程，卡维地洛可能通过下调 TNF-α、MCP-1的过表达减轻 CVB 3感染小鼠的心肌损害，起心肌保护作用。
Objective To investigate the role of tumor necrosis factor-α（TNF-α） and monocyte chemoattrac-tant protein-1 （ MCP-1 ） in the pathogenesis of viral myocarditis （VMC） and the effect of carvedilol. Methods Eighty male Balb/c mice were randomly divided into 3 groups：control group（ n = 20）, myocarditis group （n = 30） and carve-dilol group （n = 30）. The latter 2 groups were intraperitoneally inoculated with Coxsackie virus B3 （ CVB3 ） to induce VMC,while control group with virus-free Eagle＇s medium instead. Carvedilol was poured 5 mg/kg from the next day af- ter injecting virus in carvedilol group,9 g/L saline was used in control group and myocarditis group,twice a day. On the 7^th and 14^th day after inoculation,8 mice were sacrificed after taking blood sample which were randomly selected from each group. Histological cross sections of hearts were stained with hematoxylin-eosin （ HE）, and the content of serum TNF-α was assayed with enzyme-linked immunoadsordent assay, while the expression of MCP-1 mRNA in myocardial tissue was detected with reverse transcriptase-polymerase chain reaction（RT-PCR） method. Results The pathological score of myocarditis group was significantly higher than that in control group（P 〈0.01 ） ,while carvedilol group was sig-nificantly lower than that in myocardial group（P 〈 0.05 ）. The serum level of TNF-α of VMC mice in myocarditis was significantly higher than that in control group （ P 〈 0.01 ） and positively correlated with the pathological score of the myocardium （ r = O. 42, P 〈 0.05 ）, and serum TNF-α in carvedilol group was remarkably decreased compared with myo- carditis group（P 〈0.05）. The expression level of MCP-1 mRNA in myocarditis group was increased significantly com-pared with control group （P 〈 0.01 ）, and remarkably decreased in carvedilol group compared with myocarditis group （ P 〈 0.05 ）. Conclusions TNF-α and MCP-1 may be involved in the pathogenesis of VMC, and carvedilol may protect myoc
出处
《中华实用儿科临床杂志》
CAS
CSCD
北大核心
2013年第13期1020-1022,共3页
Chinese Journal of Applied Clinical Pediatrics
