摘要
目的研究IL-35在柯萨奇病毒B3(CVB3)诱导的病毒性心肌炎小鼠模型心脏中的表达意义。方法通过CVB3感染BALB/c小鼠建立病毒性心肌炎模型为感染组(IF组),给予IL-35治疗的小鼠为IL-35治疗组,感染后给予空载质粒治疗的小鼠为p-FC组,正常组未给予病毒感染并给予生理盐水为对照,感染者为酶联免疫吸附法检测心脏IL-35表达,流式细胞技术检测Th17比例,小鼠心脏组织HE染色后进行心肌炎评分。结果感染CVB3病毒1 d、3 d、5 d和7 d后,BALB/c小鼠体重逐步下降分别为(22.3±4.2)g、(18.3±2.6)g、(17.1±2.4)g和(13.2±1.8)g,心肌炎评分逐步升高分别为0.5、1.5、3.0和4.0,腹腔注射IL-35表达质粒可缓解小鼠体重和心肌炎评分;心脏IL-35表达水平与小鼠体重减轻重量(r=-0.532,P=0.034)和心肌炎评分(r=-0.670,P=0.005)呈负相关;CVB3感染第7 d,经IL-35治疗的小鼠脾脏Th17细胞比例下降(P<0.05)。结论在心脏组织表达的IL-35可能通过抑制脾脏Th17细胞治疗柯萨奇病毒B3诱导的病毒性心肌炎。
Objective To study the expression of IL-35 in the heart of Coxsackie virus B3 （CVB3） -induced viral myocarditis model. Methods BALB / c mice were infected with CVB3 to establish a model of viral myocarditis as an infection group （IF group）. IL-35-treated mice were treated with IL-35 after infection. Mice infected with plasmid-free plasmids were infected with p-FC group, The expression of IL-35 was measured by enzyme-linked immunosorbent assay （ELISA）, and Th17 ratio was measured by flow cytometry. Myocardities score was assessed after HE staining. Results The body weight of BALB / c mice decreased gradually （22.3±4.2） g, （18.3±2.6） g, （17.1±2.4） g and （13.2±1.8） g respectively after 1, 3, 5 and 7 days of CVB3 infection. Myocarditis score gradually increased 0.5 points, 1.5 points, 3.0 points and 4.0 points, respectively, and intraperitoneal injection of IL-35 expression plasmid can alleviate the mouse weight and myocarditis score increased. There was a negatively correlated between the expression of IL-35 and the body weight of BALB/c mice （r=-0.532, P=0.034）, myocardities score （r=-0.670, P=0.005）. At the 7th day of CVB3 infection, the ratio of Th17 cells in spleen of mice treated with IL-35 was significantly decreased （P〈0.05）. Conclusions IL-35 expression in cardiac tissue may be due to inhibition of splenic Th17 cells in the treatment of coxsackievirus B3-induced viral myocarditis.
出处
《中国循证心血管医学杂志》
2018年第2期218-220,223,共4页
Chinese Journal of Evidence-Based Cardiovascular Medicine
