摘要
利用亲和毛细管电泳(Affinity Capillary Electrophoresis,ACE)建立有机酸类化感物质与血清白蛋白(Bovine serum albumin,BSA)结合反应的分析方法。模拟典型有机酸类化感物质与血清白蛋白的结合反应,构建配体(有机酸)-受体(BSA)相互作用体系,采用ACE法研究不同浓度柠檬酸(Citric Acid,CA)/磺基水杨酸(Sulfosalicylic acid,SA)与BSA的结合反应机制并比较不同有机酸作用机理异同。结果表明,有机酸类化感物质CA/SA与BSA发生结合反应形成复合物CABSA和SA-BSA。依据有效淌度变化,理论方程非线性拟合结合反应的表观结合常数KCA-BSA=(1.82±0.11)×104L·mol-1、KSA-BSA=(2.12±0.12)×104L·mol-1,结合反应均为快平衡反应。相关工作阐明了血清蛋白输运有机酸类化感物质的生理作用,为化感物质与生物大分子结合反应的深入研究提供相应理论参考。
Affinity capillary electrophoresis（ ACE） had been used to establish the analytical method of binding reactions between or-ganic acids allelochemicals and bovine serum albumin（BSA）. The binding mechanisms of citric acid（CA）/sulfosalicylic acid（SA） and BSA were studied by simulating and constructing interaction system of ligand（ organic）-receptor（ BSA） ,and then the similari-ties and differences of the binding mechanism between different organic acids and different concentrations was compared. The results showed that the combined reactions of CA/SA with BSA were reacted to form CA-BSA and SA-BSA compounds. The mean apparent competition binding constants（KCA-BSA=（1. 82±0. 11）×104 L·mol-1,KSA-BSA=（2. 12±0. 12）×104L·mol-1）which was based on the changes of the effective mobility and determined through theoretical equation showed that the binding reactions of CA/SA-BSA were fast equilibrium reactions. The research results have illustrated the physiological effects of serum protein transporting organic acids allelochemicals and provided a theoretical reference for in-depth studying of the combined reaction of allelochemicals with biological macromolecules.
出处
《化学研究与应用》
CAS
CSCD
北大核心
2015年第5期635-641,共7页
Chemical Research and Application
基金
国家自然科学基金项目(20877072)资助
浙江科技厅公益项目(2012C37055)资助
关键词
有机酸类化感物质
牛血清白蛋白
ACE法
表观结合常数
organic acid allelochemicals
bovine serum albumin
affinity capillary electrophoresis
apparent binding constant
