摘要
采用简单高效的分子印迹膜技术,制备了对羟基苯甲酸(PHA)分子印迹传感器。敏感膜采用循环伏安法(CV)在玻碳电极表面合成,采用CV、微分脉冲伏安法、线性扫描伏安法和电化学交流阻抗法在K3[Fe(CN)6]/K4[Fe(CN)6]溶液中测试此分子印迹传感器的性能。在最优条件下,PHA检测线性范围为0.6～8.0μmol/L,检测限(S/N=3)为0.2μmol/L。实验结果表明:该分子印迹传感器灵敏度高、选择性好并且具有良好的再生性能,传感器对与PHA结构相似的分子有较强的抗干扰能力,可望用于植物体分泌化感物质PHA的实时监测。
A simple and effective procedure based on molecularly imprinted polymer（MIP） was developed for preparing a selective p-hydroxybenzoic acid （PHA） sensor. The sensitive layer was prepared by eleetropolymerization of o-phenylenediamine on a glassy carbon electrode（GCE） in the presence of PHA as a template. Under the optimum conditions, PHA could be determined quantitatively in the range of 0.6~ 8. 0 μmol/L with a detection limit of 0. 2 μmol/L （S/N=3）. The MIP sensor showed high selectivity,sensitivity and reproducibility for the detection of PHA, which could be used to monitor the release of PHA by plant in real time.
出处
《分析科学学报》
CAS
CSCD
北大核心
2012年第4期469-472,共4页
Journal of Analytical Science
基金
国家自然科学基金(No.31070403)
关键词
分子印迹聚合膜
对羟基苯甲酸
电化学聚合
邻苯二胺
Molecularly imprinted polymer
p-Hydroxybenzoic acid
Sensor
Electropolymerization
o-Pheny-lenediamine
