摘要
目的 了解基孔肯雅病毒（Chikungunya virus,CHIKV）毒株的时间、地区及基因型的分布情况,不同时间和地区分离毒株的相似性,核苷酸位点的变异情况以及病毒的遗传进化趋势,为基孔肯雅热的预防和控制奠定基础。方法 对美国国立生物技术信息中心（NCBI）中收录的CHIKV毒株全基因组序列共133条用DNAStar7.1软件进行核苷酸和氨基酸相似性比较及变异分析,用Mega6.06进行遗传进化分析。结果 CHIKV组内核苷酸序列相似性在97.7%～100.0%之间,CHIKV组内氨基酸序列相似性在80.3%～100.0%之间,组内相似性较高,变异率较低;CHIKV碱基间的转换较为常见占碱基突变的81.02%;分离时间、地区相近的毒株遗传距离较近,不同谱系的东/中/南非型毒株已蔓延至欧亚各地区。结论 CHIKV毒株存在一定的变异,但具有较高的遗传稳定性。
Objective Chikungunya virus （CHIKV） has caused large outbreaks worldwide in recent years. The aim of the study was to understand the time, area, variation and genetic evolution of CHIKV prevalance, and lay the foundation for prevention and control of Chikungunya fever. Methods Sequences were assembled with the DNA-Star software package and compared with ClustalX to previously published CHIKV sequences in GenBank. Neighbor-joining trees were constructed using MEGA version 6. 06. Results All strains are highly related, with 97.7% -100. 0% nucleotide sequence similarity, with 80. 3%-100. 0% amino acid similarity. Phylogenetic analysis demonstrated that the sequences were clustered into three genotypes. Viruses isolated in the same area and time were highly similar. Conclusions Although CHIKV strains vary, individual isolates are genetically conserved.
出处
《中华疾病控制杂志》
CAS
CSCD
北大核心
2015年第5期492-495,499,共5页
Chinese Journal of Disease Control & Prevention
基金
国家自然科学基金(81373050)
