摘要
蛋白质复性工艺的研究一直是重组蛋白药物研发领域的热点。稀释复性法和透析复性法的蛋白损失较大、复性得率不理想,而层析柱可以完成复性同时纯化,并能在高蛋白浓度条件下得到较高的复性率,有利于规模放大,是近年来最受关注的复性工艺。就层析柱复性工艺进行了归纳,包括凝胶过滤层析、离子交换层析、亲和层析、疏水相互作用层析的复性,并对其复性原理及各自的优缺点进行了比较分析,最后从复性工艺的有效性、优越性以及对于规模化生产的适用性三个角度论述复性工艺的评价方法。
Refolding process of proteins is a hot spot in the area of recombinant protein drug. Dilution or dialysis requires a large amount of refolding buffer and results in a low yield. Liquid chromatographic refolding has successfully proven its capability to achieve a high yield at high protein concentrations and it is easy for productive scaling up. Partial purification of recombinant protein could also be achieved simultaneously. The recent development of liquid chromatographic refolding process is reviewed in this paper. The principles, advantages and disadvantages are discussed with respect to various chromatographic refolding processes, including size exclusion chromatography, ion exchange chromatography, affinity chromatography and hydrophobic interaction chromatography. The refolding process evaluation procedures are summarized, including characterization of refolding proteins, recovery yield and industrial viability.
出处
《中国生物工程杂志》
CAS
CSCD
北大核心
2015年第3期84-91,共8页
China Biotechnology
关键词
蛋白质
层析柱
复性
工艺评价
Proteins
Liquid chromatography
Refolding
Process evaluation
