摘要
The synthesis of packing of oxygen-ethane as end-ligand bonded to silica andseparation for recombinant human interferons(rhIFN) in high-performance hydrophobicinteraction chromatography has been studied in this paper. It was showed that the packingsynthesized was not only suitable for separating standard proteins, but also for different rhIFNs,i. e., rhIFN-αA expressed in yeast, rhIFN-αA and rh1FN-γ in E. colt. The purify of threerhIFNs purified by one-step HPHIC, which rhIFN-γ expressed in E. colt was recognized as80%, rhIFN-αA expressed in yeast as 70%, and rhIFN-αA expressed in E. colt as 30%, wasmore than that of other chromatography besides affinity chromatography.
The synthesis of packing of oxygen-ethane as end-ligand bonded to silica andseparation for recombinant human interferons(rhIFN) in high-performance hydrophobicinteraction chromatography has been studied in this paper. It was showed that the packingsynthesized was not only suitable for separating standard proteins, but also for different rhIFNs,i. e., rhIFN-αA expressed in yeast, rhIFN-αA and rh1FN-γ in E. colt. The purify of threerhIFNs purified by one-step HPHIC, which rhIFN-γ expressed in E. colt was recognized as80%, rhIFN-αA expressed in yeast as 70%, and rhIFN-αA expressed in E. colt as 30%, wasmore than that of other chromatography besides affinity chromatography.
出处
《高等学校化学学报》
SCIE
EI
CAS
CSCD
北大核心
1997年第9期1462-1464,共3页
Chemical Journal of Chinese Universities
基金
军队医药卫生科研基金
关键词
高效
疏水作用色谱
干扰素
HPHIC
填料
人干扰素
High-performance hydrophobic interaction chromatography, Purification,Recombinant human interferon
