摘要
目的 :探讨静电纺壳聚糖(CHS)-聚己内酯(PCL)纳米纤维膜对骨缺损再生作用。方法 :通过静电纺丝技术制备壳聚糖-聚己内酯纳米纤维膜,以纤维膜作为载体,根据碱性磷酸酶活性和Western免疫印迹实验结果,探讨纤维膜对细胞成骨分化能力的影响。采用SPSS17.0软件包对数据进行统计学处理。结果:静电纺壳聚糖-聚己内酯纳米纤维膜对细胞成骨分化能力的作用显著高于对照组(不含材料组)(P<0.05)。结论:静电纺聚己内酯-壳聚糖纳米纤维膜在体外能够促进细胞的成骨分化,为骨缺损及再生修复提供了科学依据。
PURPOSE： To explore the effect of electrospining chitosan/polycaprolactone nano fiber membrane on regeneration of bone defects in vitro. METHODS： Chitosan/ polycaprolactone nanofiber membrane was prepared by electrospinning technique. According to alkaline phosphatase activity and Western blot results, the ability of osteogenic differentiation of cells by fiber membrane as carrier was evaluated. The data was analyzed statistically with SPSS17.0 software package. RESULTS： The osteogenic differentiation capacity of electrospining chitosan/polycaprolactone nano fiber membrane was significantly higher than the control （without materials）, and the difference was significant （P〈0.05）. CONCLUSIONS： Electrospinning chitosan/polycaprolactone nano fiber membrane can promote osteogenic differentiation of cells in vitro, which provides a scientific basis for clinical regeneration of bone defects.
出处
《上海口腔医学》
CAS
CSCD
2015年第2期129-134,共6页
Shanghai Journal of Stomatology
基金
国家自然科学基金(81201191)
安徽医科大学博士科研基金(XJ201108)~~
关键词
静电纺丝
壳聚糖
聚己内酯
成骨分化
Electrospinning
Chitosan
Polycaprolactone
Osteogenic differentiation
