摘要
目的:研究复合聚己内酯(PCL)静电纺丝膜的制备,比较3种纺丝膜的细胞相容性。方法:通过加入胶原(COL)和羟基磷灰石(HA),并按一定比例制成纯PCL膜、PCL+5%COL膜和PCL+5%COL+1%HA膜。扫描电镜观察膜片形貌,接触角实验检测材料亲水性。MTT法检测牙周膜细胞在3组膜片上的增殖情况。活-死细胞染色法比较牙周膜细胞与膜片复合培养9 d时细胞存活情况。DAPI染色观察牙周膜细胞在3组纺丝膜上的分布情况。结果:扫描电镜结果显示,纯PCL膜纤维表面光滑平整,PCL+5%COL膜纤维表面存在微小凹坑,PCL+5%COL+1%HA膜纤维表面有突起结节,结节表面光滑连续。PCL+5%COL+1%HA膜水接触角显著小于纯PCL膜和PCL+5%COL膜。牙周膜细胞在PCL+5%COL+1%HA膜上的细胞增殖和活性显著优于其他两种膜片(P<0.01),细胞分布更密集。结论:将COL、HA与PCl混纺,可以获得在微观形貌上与纯PCL纺丝膜存在差异的纳米纤维膜。PCL+5%COL+1%HA膜表现出较好的细胞相容性。
Objective：The study aimed to fabricate electrospinning membranes with PCL,COL and HA,and test their biocompatibility with periodontal ligament cells（PDLCs）. Method：Three electrospinning membranes were fabricated with different spinning solution composition of PCL,PCL ＋5 % COL and PCL ＋5 % COL ＋1 % HA,respectively. The membrane morphology was observed through scan electron microscope and the hydrophilic performance was tested by contact angles.MTT was applied to investigate the proliferation of PDLCs seeded on the membranes and the viability was measured by live and dead staining method. DAPI staining was used to observe the distribution of PDLCs co-cultured with membrane. Result：All the three types of membranes were fabricated with uniform nano-fibers. The fibers of PCL＋5 %COL membrane exhibited concave surface and that of PCL＋5 %COL＋ 1 %HA membrane raised nodular surface with smooth and continuous surface. PCL＋5 %COL＋ 1 %HA membrane had the smallest contact Angles rank among the three membranes. The proliferation and viability of PDLCs on the PCL ＋5 % COL ＋ 1 % HA membrane were significantly higher than those on other two membranes（P〈0.01）. Additionally,cells on the PCL＋5 %COL＋1 %HA membrane was more intensive than other two groups（P〈0.01）. Conclusion：The composition solution of COL,HA and PCL can change the morphology of electrospinning membranes. Among them,the PCL＋5 %COL＋1 %HA electrospinning membrane suggested good biocompatibility.
出处
《临床口腔医学杂志》
2016年第5期281-284,共4页
Journal of Clinical Stomatology
关键词
静电纺丝
牙周膜细胞
微观形貌
细胞相容性
Electrospinning
Periodontal ligament cells
Morphology
Cell compatibility
