摘要
目的:采用响应面分析法(RSM)对绞股蓝总黄酮的提取工艺进行优化。方法:以总黄酮提取率为考察指标,在单因素试验基础上选择试验因素和水平,进行Box-Behnken响应面试验设计,得到最佳操作条件及二次方程响应面模型。结果:绞股蓝总黄酮提取的最佳工艺条件为:乙醇体积分数71%,料液比1∶14,超声时间32 min,该工艺条件下,绞股蓝总黄酮提取率模型预测值为4.676%,验证试验得率为4.641%。结论:回归模型拟合度良好,该提取工艺可行、可靠。
Objective:To optimize the extraction process of total flavonoids in Gynostemma pentaphyllum by the method of response surface analysis ( RSM) . Methods: Based on the choice of factors and levels according to the results of single factor test, Box-Be-hnken response surface experiment was designed with the extraction rate of total flavonoids as the index to obtaln the best operation con-ditions and two equations of the response surface model. Results: The optimal extraction conditions of total flavonoids in Gynostemma pentaphyllum were as follows:the volume fraction of ethanol was 71%, the ratio of solid to liquid was 1∶14, and the ultrasonic time was 32 min. Under the conditions, the model predictive value of extraction rate of total flavonoids in Gynostemma pentaphyllum was 4. 676%, and the extraction rate in the verification test was 4. 641%. Conclusion:The fit of the regression model is good, and the ex-traction technology is feasible and reliable.
出处
《中国药师》
CAS
2015年第5期714-717,共4页
China Pharmacist
基金
国家星火计划项目(编号:2012GA740049)
关键词
绞股蓝
黄酮
提取工艺
响应面分析法
Gynostemma pentaphyllum
Flavonoids
Extraction technology
Response surface analysis