摘要
目的:探讨叉状头转录因子(FoxP3)/T细胞免疫球蛋白黏蛋白分子-3(Tim-3)信号通路中的相关基因在系统性硬化症(SSc)中的表达。方法:收集我院30例系统性硬化症患者和15例健康对照的外周血,分离得到外周血单核细胞(PBMCs),流式细胞术检测PBMCs中CD4+CD25+Treg细胞和Th17辅助细胞(Th17)变化,RT-PCR检测PBMCs中FoxP3和Tim-3mRNA变化,ELISA检测细胞毒T淋巴细胞相关抗原4(cytotoxic T lymphocyte-associated antigen-4,CTLA-4)及γ干扰素(IFN-γ)含量。结果:与健康对照组相比,SSc患者PBMCs中Treg细胞和Th17细胞数目上升,但二者比值下降,FoxP3mRNA表达量下降,而Tim3mRNA表达量上升;CTLA-4及IFN-γ含量皆下降。结论:SSc患者PBMCs中T调节细胞(Treg)和Th17水平提高,但功能上存在缺陷,可能与FoxP3/Tim-3信号通路中相关基因表达变化有关。
Objective： To explore the molecular of immunology of FoxP3/Tim-s signaling pathway related gene in systemic sclerosis （SSc）. Methods.. Peripheral blood was collected from 30 SSc patients and 15 healthy cases. The ratio of CD4^＋ CD25^＋ Treg and Th17 were measured by flow cytometry. The FoxP3 and Tim 3 mRNA were detected by RT-PCR. The level of CTLA-4 and IFN-）＇ was determined by elisa. Results： The cell number of CD4^＋ CD25^＋ Treg and Thl7 from peripheral blood mononuclear cells （PBMCs） of SSc patients was higher than the healthy group, but the ratio of Treg/Th17 was lower than the healthy group. Compared with the healthy group, the expression of FoxP3 from PBMCs of SSc patients was decreased, the ex- pression of Tim-3 from PBMC of SSc patients was increased. The level of CTLA-4 and IFN y from PBMC of SSc patients was lower than the healthy group, Conclusions： The levels of Treg and Th17 from PBMCs of SSc patients are increased, but defects exist on the function, which may be associated with FoxP3/Tim 3 signaling pathway related gene changes.
出处
《海南医学院学报》
CAS
2015年第6期735-738,共4页
Journal of Hainan Medical University
基金
湖北省教育厅自然科学研究项目(B20121242)~~
