摘要
目的探讨中国汉族人群中肿瘤免疫调节基因TIM-3启动子区单核苷酸多态性与胃癌的遗传易感性关联。方法提取190例胃癌患者及216例健康人群外周血基因组DNA,用PCR-RFLP方法检测2组人群中TIM-3基因启动子区-574G/T、-882C/T、-1516G/T3个多态性位点的基因型;各多态位点的基因型与胃癌风险之间的相关性以OR值(odds ratio)及其95%CI(confidence intervals)表示;OR值及其95%CI以非条件Logistic回归模型分析,均经各混杂因素校正后取得;所有的统计检验均为双侧概率检验,统计学分析使用SPSS11.5软件。结果 PCR-RFLP检测结果显示,TIM-3启动子区的3个多态性位点在检测人群中均存在,但3个位点的多态纯合形式在研究人群中几乎没有出现。190例胃癌患者与216例健康对照人群中3个多态性位点的基因率差异均有统计学意义(P<0.05)。经混杂因素的校正后,Logistic回归分析结果显示3个位点的基因型差异均有统计学意义,-574G/T位点:OR=3.92(95%CI:1.40-10.94);-882C/T位点:OR=3.20(95%CI:1.22-8.41);-1516G/T位点:OR=2.32(95%CI:1.23-4.40),P值均<0.05。结论 TIM-3启动子区的基因多态性对于胃癌的发生有着显著的易感关联,虽然该3个位点的多态性纯合基因型存在形式极低,但结果依然提示对于胃癌的发生,TIM-3启动子区的多态性仍是一个重要的易感危险因素。
Objective This study was performed to elucidate whether the polymorphisms within the tumor immunity-regulated gene TIM-3 promoter region were associated with the risk of gastric cancer in the Han ethnic group of China. Methods The association was analyzed in a hospital-based case-control study of 190 gastric cancer cases and 216 cancer-free controls. DNA was obtained from blood samples and the polymorphisms of-574G/T,-882C/T,and-1 516G/T within TIM-3 gene promoter region were genotyped by the method of PCR-RFLP. The correlation between the risk of gastric cancer with the genotype of the polymorphisms was assessed by the odds ratio（OR） values and their 95% confidence intervals（CI）. Unconditional Logistic regression model adjusted by the confounding factors was used to analyze the statistical association of the genotypes in two groups by using the SPSS 11.5 software. Results The results of PCR-RFLP showed that the three polymorphisms in TIM-3 gene promoter region were present in our study population. But the homozygous form of the polymorphisms almost did not appear. Statistical analysis revealed that the allele frequency of the three polymorphisms displayed significant difference between the gastric cancer patients and healthy controls（P0.05）. Logistic regression analysis adjusted for confounding factors exhibited that all the three loci genotypes possessed statistically significant difference（P0.05）：loci-574G/T（OR=3.92,95% CI：1.40-10.94）,loci-882C/T（OR=3.20,95% CI：1.22-8.41）,and loci-1 516G/T（OR=2.32,95% CI：1.23-4.40）,respectively. Conclusion Although the three polymorphic homozygous genotypes were very rare,the results still revealed that the polymorphisms within TIM-3 gene promoter region were significantly contributed to the genetic susceptibility of gastric cancer in the Han ethnic group of China.
出处
《首都医科大学学报》
CAS
北大核心
2010年第3期299-303,共5页
Journal of Capital Medical University
基金
国家高技术研究发展计划(863计划)项目(2007AA02Z4Z4)~~
关键词
TIM-3基因
胃癌
单核苷酸多态性
易感性
TIM-3（T cell immunoglobulin domain and mucin domain-3）
gastric cancer
single nucleotide polymorphism
susceptibility
