摘要
目的:探讨天麻素在体外对人胃癌细胞SGC-7901细胞活力和凋亡的影响。方法:培养人胃癌细胞株SGC-7901,随机分为正常组、对照组和实验组,再按不同时间点分为6、12、24、48、72 h 5个亚组,每个亚组8孔细胞。实验组按2 mg/m L加入天麻素,对照组加入等量生理盐水,正常组不作其它处理。运用MTT法检测不同时间点细胞活力,运用TUNEL法检测细胞凋亡情况。结果:天麻素对人胃癌细胞SGC-7901具有增殖抑制和促进凋亡作用,并具有时间依赖关系。正常组、对照组之间细胞活力及凋亡比较均无统计学意义(P>0.05)。在6 h、12 h、24 h、48 h、72 h时间点,实验组的细胞活性分别为0.41±0.05、0.42±0.07、0.43±0.06、0.47±0.07、0.52±0.08,对照组分别为0.44±0.07、0.49±0.12、0.50±0.09、0.69±0.11、0.72±0.12,实验组细胞活力较对照组降低(P<0.05),实验组的凋亡指数分别为7.12±0.65、10.48±1.26、15.37±1.69、17.52±2.33、33.62±3.65,对照组的凋亡指数分别为1.08±0.05、3.41±0.56、6.09±0.91、9.52±1.14、11.57±1.18,实验组较对照组凋亡显著增加(P<0.05)。结论:天麻素可降低人胃癌SGC-7901细胞的活力并增加细胞的凋亡,可能具有抗肿瘤的作用。
Objective： To explore the effect of Gastrodin on cell viability and apoptosis of SGC-7901 in vitro. Methods： SGC-7901 cells were cultured and then were randomly divided into normal, control and experimental groups. According to different effecting time, they were further divided them into 6, 12, 24, 48, 72 h, 5 subgroups. Each subgroup was given 8 well cells. The experimental group received 2 mg/mL Gastrodin, control group was given saline, normal group had no other treatment. The cell viability was detected in different time points by MTT, TUNEL method was used for apoptosis detection. Results： Gastrodin inhibited the cell proliferation and promoted cell apoptosis, and these effects had time dependency. Cell viability and apoptosis between normal and control group had no statistical significance （P〉0.05）. At 6 h, 12 h, 24 h, 48 h, 72 h time points, the cell viability of experimental group was respectively 0.41± 0.05, 0.42± 0.07, 0.43± 0.06, 0.47± 0.07, 0.52± 0.08, while that was respectively 0.44± 0.07, 0.49± 0.12, 0.50± 0.09, 0.69± 0.11, 0.72± 0.12 in control group. The cell viability of experimental group was lower than that of control group （P〈0.05）. The apoptotic index of experimental group was respectively 7.12± 0.65, 10.48± 1.26, 15.37± 1.69, 17.52± 2.33, 33.62± 3.65, while that was respectively 1.08± 0.05, 3.41± 0.56, 6.09± 0.91, 9.52± 1.14, 11.57± 1.18 in control group. Compared with control group, the apoptotic index of experi- mental group was profoundly higher （P〈0.05）. Conclusion： Gastrodin can decrease the gastric cancer SGC-7901 cell viability and in- crease cell apoptosis, which may have anti-tumor effect.
出处
《现代生物医学进展》
CAS
2015年第13期2421-2424,共4页
Progress in Modern Biomedicine
基金
国家自然科学基金项目(81260075
31260253)
云南省科技厅-昆明医科大学应用基础研究联合专项(2012FB002
2012FB092
2013FB114
2013FZ295)
云南省肿瘤转化医学工程技术研究中心(2011DH011)
云南省教育厅科学研究基金项目(2013J24C)
