摘要
目的:研究万寿菊茎叶中两种黄酮类化合物4'-甲氧基-泽兰素-3-O-β-D-葡萄糖苷(化合物Ⅰ)和山柰酚-3,7-O-α-L-双鼠李糖苷(化合物Ⅱ)的抗肿瘤活性。方法:人胃癌细胞SGC7901与人肝癌细胞SMMC7721经20,40,80,120,160μmol.L-1的两种化合物作用24,48,72 h后,使用MTT法测定抑制率,并以5-氟脲嘧啶作为阳性对照。使用吖啶橙/溴化乙锭双荧光染色法观察浓度为160μmol.L-1的两种化合物作用于细胞密度为1×106个/mL的2种肿瘤细胞48 h细胞形态学变化及细胞凋亡情况。结果:不同浓度的两种黄酮类化合物均可抑制人胃癌细胞SGC7901和人肝癌细胞SMMC7721的增殖,且呈现浓度与时间依赖性。化合物Ⅰ作用这2种癌细胞48 h的半数抑制浓度(IC50)分别为111.7,330.4 mg.L-1。化合物Ⅱ作用这两种癌细胞48 h的IC50分别为683.8,464.7 mg.L-1。2种化合物作用肿瘤细胞后细胞形态发生改变,并有部分细胞发生凋亡。结论:万寿菊茎叶中分离的2种黄酮类化合物具有体外抗肝癌和胃癌活性。
Objective： To study the anti-cancer activity of two flavonoids 4＇-methoxy-eupatolitin-3-O-βD-glucoside（compoundⅠ） and kaempfrol-3,7-O-α-L-di-rhamnoside（compound Ⅱ） in the leaf and stem of Tagetes erecta.Method： SGC7901 and SMMC7721 cells were treated by 20,40,80,120,160 μmol.L-1 two flavonoids for 24,48,72 h,MTT assay was used to determine the inhibition rates,and 5-FU was used as positive control.AO / EB double fluorescent staining was used to observe the changes of cell morphology and the apoptosis of tumor cells at the concentration of 1 × 106/ mL after treated with 160 μmol.L-1 of two flavonoids for 48 h.Result： The different concentrations of two flavonoids could both inhibit the proliferation of SGC7901 and SMMC7721 in the time-and dose-dependent manner.The IC50 of compoundⅠ was 111.7 mg.L-1 and 330.4 mg.L-1,respectively.The IC50 of compound Ⅱ was 683.8 mg.L-1 and 464.7 mg.L-1,respectively.The morphology of two tumor cancer cells was changed after treated by two compounds,and part of them emerged apoptosis.Conclusion： Two flavonoids separated from the leaf and stem of T.erecta have the activity of anti-liver cancer and anti-gastric cancer in vitro.
出处
《中国实验方剂学杂志》
CAS
北大核心
2013年第13期233-237,共5页
Chinese Journal of Experimental Traditional Medical Formulae
关键词
万寿菊
黄酮
MTT法
荧光染色法
Tagetes erecta
flavonoid
MTT
fluorescence staining
