摘要
目的:观察消胀方对四氯化碳(CCl4)诱导的大鼠肝纤维化的防治作用。方法:Wistar雄性大鼠66只随机分为6组,分别为正常组、CCl4模型组、秋水仙碱组、消胀方低、中、高剂量组,除正常组外,均采用CCl4诱导大鼠成肝纤维化模型,造模开始时分别给予秋水仙碱1 mg·kg-1,消胀方200,400,800 mg·kg-1ig 7周。观察大鼠体重和肝脾指数的变化;采用HE染色和天狼猩染色观察肝组织病理及胶原沉积情况;检测血清丙氨酸氨基转移酶(ALT),门冬氨酸氨基转移酶(AST),血清透明质酸(HA),层黏蛋白(LN),Ⅲ型前胶原(PCⅢ);检测肝组织羟脯氨酸(Hyp),超氧化物歧化酶(SOD),谷胱甘肽过氧化物酶(GSH-Px),丙二醛(MDA)含量。结果:与正常组比较,模型组大鼠的体重明显下降(P<0.05),大鼠肝脏指数和脾脏指数均明显增高(P<0.01),大鼠血清ALT,AST,HA,LN,PCⅢ水平明显升高(P<0.01),大鼠肝组织Hyp,MDA含量明显升高(P<0.01),SOD和GSH-Px活性明显降低(P<0.01);与模型组比较,秋水仙碱和消胀方组ALT,AST,HA,LN,PCⅢ均明显下降(P<0.01,P<0.05),Hyp,MDA含量明显下降(P<0.01,P<0.05),SOD和GSH-Px活性明显升高(P<0.01,P<0.05);肝组织HE染色显示模型组大鼠肝脏肝细胞明显变性坏死,肝小叶结构破坏,假小叶形成,肝间质大量炎性细胞浸润;秋水仙碱和消胀方组肝组织假小叶数目减少,炎性细胞浸润减少;天狼猩红染色显示模型组大鼠肝窦周围胶原沉积明显;秋水仙碱和消胀方组胶原纤维明显减少。结论:消胀方对CCl4诱导的大鼠肝纤维化有一定的防治作用,其机制可能与保护肝细胞、减轻肝脏炎症和抗脂质过氧化损伤有关。
Objective： To investigate the effect of Xiaozhang recipe （XR） on liver fibrosis （LF） induced by carbon tetrachloride （CC14） in rats. Method： LF model was induced by injecting CC14 subcutaneously twice weekly for seven weeks and the rats were divided into the normal group, the model group, the colchicine group （1 mg·kg^-1）, the low-, middle-and high-dose XR groups （200, 400, 800 mg·kg^-1）. All rats were intragastrically administrated with the corresponding medicines for seven weeks. The change of body weight and the index of liver and spleen were observed. Histological changes and collagen deposition were observed by HE and Sirius red staining sections. Serum samples were collected and alanine aminotransferase （ALT）, aspartate aminotransferase （AST） , hyaluronidase （HA） , laminin （LN） , Type Ⅲ procollagen （PC Ⅲ） were assayed. Liver tissues were harvested and hydroxyproline （Hyp） , superoxide dismutase （SOD） , glutathione peroxidase （GSH- Px） and maleic dialdehyde （MDA） were detected. Result： Compared with the normal group, the body weight decreased, the index of liver and spleen increased, serum levels of ALT, AST, HA, LN, PC Ⅲ and hepatic Hyp, MDA increased, hepatic SOD and GSH-Px decreased in the model group （P 〈 0.01, P 〈 0.05）. Compared withthe model group, serum levels of ALT, AST, HA, LN, PC Ⅲ and hepatic Hyp, MDA decreased, hepatic SOD and GSH-Px increased in the colchicine and XR groups （P 〈 0.01, P 〈 0.05 ）. Histological detection showed serious necrosis, false lobule, inflammatory cell infiltration and collagen deposition in the liver of model group and these pathological changes could be alleviated by colchicine and XR. Conclusion： XR has preventive and therapeutic effects on liver fibrosis induced by CC14 by inhibiting hepatic lipid peroxidation and alleviating the inflammation.
出处
《中国实验方剂学杂志》
CAS
CSCD
北大核心
2015年第9期102-107,共6页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家“重大新药创制”科技重大专项(2012ZX10005004-002,2012ZX-10005010-002-003)
国家自然科学基金项目(81102570)
关键词
肝纤维化
四氯化碳
消胀方
羟脯氨酸
脂质过氧化
liver fibrosis
carbon tetrachloride
Xiaozhang recipe
hydroxyproline
lipid peroxidation
