摘要
本文运用亚硝基胍(Nitrosoguanidine,NTG)诱变,并结合乙酰辅酶A羧化酶抑制剂喹禾灵筛选,获得了2株能够稳定遗传的高产钝顶节旋藻突变株623-8和623-16。与钝顶节旋藻出发株623相比,突变株的藻丝更长,更易聚集成团。在pH=8.5、光照强度45μmol·m-2·s-1和培养温度28℃的条件下,突变株623-8和623-16的生物量分别比出发株提高67.86%和46.43%。蛋白含量分别比出发株623提高1.98%和0.84%;脂类含量提高8.54%和4.88%;乙酰辅酶A羧化酶活性提高31.82%和11.36%。研究结果表明,以亚硝基胍诱变结合喹禾灵筛选,可筛选出生长速度快、蛋白含量高等性状优良的钝顶节旋藻藻株,在工业生产中有实用意义。
Stably inherited strains of Arthrospira platensis with high-yield trait were obtained by using nitrosoguanidine（NTG）mutagenesis and Quizalofop-Ethyl screening.Compared with the original strain623 of A.platensis,the two mutants of 623-8and 623-16 had longer filaments and easily aggregated into clusters.Moreover,higher growth rate and higher contents of protein and lipid as well as higher activities of acetyl coenzyme A carboxylase were detected.The growth rate of mutants of 623-8and 623-16 reached their maximum of 67.86% and 46.43%respectively at 28 ℃ with pH=8.5and light intensity of 45μmol·m-2·s-1.Protein contents of both mutants increased by 1.98% and 0.84% respectively.While,lipid contents increased by 8.54% and 4.88%.The activities of acetyl coenzyme A carboxylase increased by 31.82% and 11.36% respectively.Our findings showed that a combination of NTG with Quizalofop-Ethyl can be used to mutate and screen the Arthrospirastrains for high growth rate and high protein content,which is important to improve the yield and quality of Arthrospirain industry.
出处
《中国海洋大学学报（自然科学版）》
CAS
CSCD
北大核心
2015年第4期59-65,共7页
Periodical of Ocean University of China
基金
山东省优秀中青年科学家科研奖励基金项目(BS2012HZ017)
国家高技术研究发展计划项目(2008AA09Z410)资助
关键词
钝顶节旋藻
亚硝基胍
诱变
喹禾灵
Arthrospira platensis
Nitrosoguanidine
mutagenesis
Quizalofop-Ethyl
