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习惯性流产患者绒毛和蜕膜中Toll样受体4与Treg/T_H17细胞免疫平衡的相关性研究 被引量:7

Correlation between Toll-like receptor 4 and Treg/T_H17 cells in villus and deciduas of patients with recurrent spontaneous abortion
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摘要 目的探讨习惯性流产(RSA)患者绒毛和蜕膜中Toll样受体4(TLR4)与Treg/TH17细胞免疫平衡的关系。方法收集32例RSA患者和32例健康对照组的绒毛和蜕膜,用实时荧光定量PCR法检测其TLR4 mRNA表达水平,用流式细胞术检测Treg和TH17细胞数量,分析TLR4 mRNA与Treg/TH17细胞间的相关性。结果 RSA患者绒毛和蜕膜中TLR4 mRNA的相对表达水平分别为2.6±1.1、2.1±1.1,明显低于健康对照组(7.4±2.3、6.8±1.3),差异有统计学意义(t分别为10.650、15.613,P均<0.01)。Treg细胞百分率分别为(7.75±1.83)%、(7.24±1.71)%,明显低于健康对照组(12.18±2.65)%、(11.64±3.23)%,差异有统计学意义(t分别为7.781、6.810,P均<0.01);TH17细胞百分率分别为(7.78±2.13)%、(8.13±2.11)%,明显高于健康对照组(3.85±1.53)%、(4.11±1.52)%,差异有统计学意义(t分别为8.477、8.13,P均<0.01)。RSA患者绒毛和蜕膜中TLR4 mRNA水平均与Treg细胞数量呈正相关(r分别为0.925、0.948,P均<0.01);RSA患者绒毛和蜕膜中TLR4 mRNA水平均与TH17细胞数量呈负相关(r分别为-0.954、-0.939,P均<0.01)。结论 RSA患者绒毛和蜕膜中TLR4低表达与Treg/TH17免疫失衡有关,可能参与RSA的发生。 Objective To investigate the correlation between Toll-like receptor 4( TLR4) and Treg / TH17 cells in villus and deciduas of the patients with recurrent spontaneous abortion( RSA). Methods The villus and deciduas were collected from 32 patients with RSA and 32 normal pregnant women. The mRNA levels of TLR4 were determined with real-time fluorescent quantitative PCR. The percentages of Treg and TH17 cells in the villus and deciduas were measured with flow cytometry. The correlations between TLR4 mRNA and Treg / TH17 cells were analyzed. Results The relative expression level of TLR4 mRNA in villus and deciduas of RSA patients were( 2. 6 ± 1. 1) and( 2. 1 ± 1. 1) respectively,which were significantly lower than those of normal pregnant women [( 7. 4 ± 2. 3) and( 6. 8 ± 1. 3) ]( t = 10. 650 and 15. 613,P〈 0.01). The percentages of Treg cells in villus and deciduas were( 7. 75 ± 1. 83) % and( 7. 24 ± 1. 71) % respectively,which were significantly lower than those of normal pregnant women [( 12. 18 ± 2. 65) % and( 11. 64± 3. 23) % ]( t = 7. 781 and 6. 810,P〈 0.01). The percentages of TH17 cells were( 7. 78 ± 2. 13) % and( 8. 13 ± 2. 11) % respectively,which were obviously lower than those of normal pregnant women [( 3. 85 ± 1. 53) % and( 4. 11 ± 1. 52) % ]( t = 8. 477 and8. 745,P〈 0.01). There was strongly positive correlation between the levels of TLR4 mRNA and the percentages of Treg cells in villus and deciduas from RSA patients( r = 0. 925 and 0. 948,P〈 0.01),while a remarkably negative correlation between the levels of TLR4 mRNA and the percentages of TH17 cells was found in villus and deciduas of RSA patients( r =- 0. 954 and-0. 939,P〈 0.01). Conclusion The low expression of TLR4 mRNA was related to the imbalance of Treg / TH17 cells in villus and deciduas of RSA patients,which may participate in the immunopathogenesis of RSA.
出处 《临床检验杂志》 CAS CSCD 2015年第3期193-196,共4页 Chinese Journal of Clinical Laboratory Science
基金 深圳市科技计划项目(医疗卫生类)(201203194)
关键词 习惯性流产 TOLL样受体4 Treg/TH17平衡 recurrent spontaneous abortion Toll-like receptor 4 balance of Treg / TH17
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  • 1刘雨生,赵淑云,季静娟,翟志敏,童先宏,骆丽华,栾红兵.原因不明复发性流产患者调节性T淋巴细胞比例及功能的测定[J].中华妇产科杂志,2007,42(3):176-179. 被引量:12
  • 2Croy BA, Ashkar AA, Minhas K,et al. Can murine uterine natural Killer cells give insights into the pathogenesis of preeclamp sia? J Soc Gynecol Investig,2000, 7 ( 1 ) : 12 - 20. 被引量:1
  • 3Visser N, van Rijn BB, Rijkers GT, et al. Inflammatory changes in preeclampsia:current understanding of the maternal innate and adap- tive immune response. Obstct Gynecol Surv, 2007, 62 ( 3 ) : 191 - 201. 被引量:1
  • 4Wu X, Jin LP, Yuan MM, et al, Human first - trimester trophoblast cells recruit CD56bright CD16 - NK ceils into decidua by way of ex-pressing and secreting of CXCL12 ! stromal cell - derived factor 1. J immunol, 2005, 175:661 -681. 被引量:1
  • 5Pevsner - Fischer M, Morad V, Cohen - Sfady M, et al. Toll - like receptors and their ligands control mesenchymal stem cell functions. Blood, 2007, 109(4) :1422 - 1432. 被引量:1
  • 6Medzhitov R, Preston - Hurlburt P,Janeway CA. A human homologue of the drosophila Toll protein signals activation of adaptive immunity. Nature, 1997,388 (6640) :394 - 397. 被引量:1
  • 7Akira S. Mammalian Toll - like receptors. Curr Opin Immunol, 2003,15:5 - 11. 被引量:1
  • 8Crocker IP, Barratt S, Kaur M, et al. The in -vitro characterization of induce dapoptosis in placental cytotrophoblasts and syncytotropho- blasts. Placenta, 2001,22(10) :822 - 830. 被引量:1
  • 9Abrahams V M,Bole - Aldo P,Kim Y M,et al. Divergent trophoblast responses to bacterial products mediated by T LRs. J Immunol,2004, 173 (7) :4286-4296. 被引量:1
  • 10Lee HK, Iwasaki A. Innate control of adaptive immunity : dendritic cells and beyond. Sem Immunol,2007,1:48. 被引量:1

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