摘要
目的探讨外周血CD4+CD25+Treg细胞数量和抑制性细胞因子TGF-βI、L-10含量变化在反复自然流产中的作用。方法采用ELISA法检测30例反复自然流产患者3、0例正常妊娠妇女和30例正常非孕妇女外周血抑制性细胞因子TGF-β和IL-10含量,三色流式细胞术检测上述研究对象外周血CD4+CD25+T细胞比例。结果反复自然流产组妇女外周血CD4+CD25+Treg细胞的百分率(6.66±1.30)%明显低于正常非孕组(10.99±2.64)%(P<0.05)和正常妊娠组(12.63±3.05)%(P<0.05)。反复自然流产组妇女外周血TGF-β(123.64±27.31)pg/L明显低于正常非孕组对照组TGF-β(179.25±24.67)pg/L(P<0.05)和正常妊娠组TGF-β(211.72±63.49)pg/L(P<0.05);反复自然流产组妇女外周血IL-10(145.37±42.45)pg/L明显低于正常非孕组对照组IL-10(203.47±42.56)pg/L(P<0.05)和正常妊娠组IL-10(234.45±51.21)pg/L(P<0.05)。结论外周血CD4+CD25+Treg细胞的比例低下以及抑制性细胞因子TGF-β和IL-10含量降低可能参与反复自然流产的发生。
Objective To investigate the role of CD4＋CD25＋ regulatory T cells,TGF-β and IL-10 in recurrent spontaneous abortion（RSA）.Methods The levels of TGF-β and IL-10 in peripheral blood from 30 RSA patients,30 normal pregnancy women and 30 non-pregnancy women were detected by Enzyme-linked Immunosorbent Assay（ELISA）.Peripheral blood CD4＋CD25＋Treg were analyzed by flow cytometry from the same research objects.Results The percentage of CD4＋CD25＋ regulatory T cells in RSA group was lower than those in non-pregnancy group or normal pregnancy group.The levels of TGF-β and IL-10 in RSA group were all lower than those in non-pregnancy group or normal pregnancy group.Conclusiona CD4＋CD25＋ regulatory T cells,TGF-β and IL-10 may play an important role in RSA.
出处
《医药论坛杂志》
2011年第12期19-21,共3页
Journal of Medical Forum
