摘要
为了评估草坪植株的Co(Ⅱ)耐受性,本研究通过对4种草坪植物苗期的株高、地上生物量、相对电导率、叶绿素含量、可溶性蛋白、可溶性糖、游离脯氨酸、丙二醛,O2-含量等生理生化指标的测定分析,利用主成分分析法和隶属函数法对4种植物进行综合评判,建立了植株Co(Ⅱ)耐受性综合评价体系。结果表明,4种草坪植物的相对电导率、脯氨酸、丙二醛、超氧阴离子自由基等指标随着Co(Ⅱ)处理浓度的增加而升高,各处理间存在着显著差异,其他指标的规律各具特点。高羊茅的Co(Ⅱ)耐受性明显高于其他3种植物,一年生黑麦草虽然Co(Ⅱ)耐受性最低,但由于其巨大的生物量优势,也具有较大的Co(Ⅱ)污染土壤修复潜力。本研究通过建立植物Co(Ⅱ)耐受性综合评价方法,为寻找Co(Ⅱ)抗性优良植株,对土壤Co(Ⅱ)污染的植物修复提供了一定的技术手段和科学依据。
In order to evaluate the resistance to Co（ II） of lawn plant,to establish a comprehensive evaluation method for resistance to Co（ II） of lawn plant. Four kinds of lawn plant which have some growth advantages in Co（ II） polluted environment were selected as experimental materials in this research. Comprehensive evaluation index include the height,aboveground biomass, membrane permeability, chlorophyll content, soluble protein, soluble sugar, free proline,MDA,and O2-contents after Co（ II） stress. A comprehensive evaluation of resistance to Co（ II） of these lawn plants in adult stage was established by using principal component analysis and its membership function. The results showed that the comprehensive evaluation values of resistance to Co（ II） of four kinds of lawn plant,the pecking order is tall fescue,timothy grass,inflorescences,annual ryegrass. In this research,the resistance to Co（ II） of tall fescue is better than the other plants. Though the resistance to Co（ II） of the annual ryegrass is the worst on some others,the annual ryegrass plays an important part in heavy metals contaminated soil remediation,because of the advantage of its huge biomass. In this research,some pattern has been found by physiological and biochemical analysis. For four kinds of lawn plant,the values of membrane permeability,free proline,MDA,O2-contents increased as the increase of Co（ II） concentrations. For inflorescences and annual ryegrass,the values of height,aboveground biomass,chlorophyll content,soluble protein and soluble sugar significant decreased in high Co（ II） concentrations. For tall fescue,the values of chlorophyll content,soluble protein and soluble sugar increased at first and decreased later with the increase of Co（ II） concentrations. For timothy grass,the values of chlorophyll content and soluble protein increased at first and decreased later. With the increase of Co（ II） concentrations,the values of soluble sugar,by contrast,decreased at first and increased later.
出处
《核农学报》
CAS
CSCD
北大核心
2015年第4期777-785,共9页
Journal of Nuclear Agricultural Sciences
基金
国家自然科学基金资助项目(11075134)
国防科技项目资助(14ZG6101)
四川省生物质资源利用与改性工程技术研究中心基金资助(13zxsk02)
关键词
草坪植物
Co(Ⅱ)耐受性
主成分分析法
隶属函数法
植物修复
lawn grass
cobalt tolerance
principal component analysis
subordinate function values analysis
phytoremediation
