摘要
运用实验法和数理统计法探究体育运动中结缔组织生长因子对肌腱干细胞分化产生的影响。将周围结缔组织从活体中进行细胞分离及培养;取P2代细胞爬片进行免疫细胞化学检测细胞表型抗原标记CD44、CD29。取P3代细胞进行肌腱干细胞多向分化能力鉴定,收集未处理的肌腱干细胞和分化后的肌腱细胞,分别用实时荧光定量PCR和Western印迹检测I型胶原和Tenascin-C m RNA和蛋白水平的表达。实验结果表明:细胞免疫荧光染色CD44、CD29阳性,表明我们分离提取的组织细胞为肌腱干细胞;肌腱干细胞可以向脂肪细胞、骨细胞、软骨细胞分化;分化后的肌腱细胞,I型胶原的m RNA水平含量升高了5.3倍,Tenascin-C升高了2.1倍(P<0.01),I型胶原的蛋白表达升高了3.3倍,Tenascin-C的表达升高了1.8倍(P<0.05)。结论:结缔组织生长因子可以促进肌腱干细胞分化。
To investigate the effect of Connective tissue growth factor（CTGF） on the differentiation of tendon stem/progenitor cells（ TSPCs） in sports, this paper uses the experimental method and mathematical statistics method. Cells were isolated from the surrounding connective tissue of the living; the protein expressions of CD44、CD29 were detected by immunocy to chemistry staining from the cells at passage 2. Cells at passage 3were used to demonstrate the multi-differentiation potential of the TSPCs, the m RNA expression of tenascin C and collagen type I were determined by real-time PCR, and the protein expressions of tenascin C and collagen type I were determined by Western blot. Results indicates that immunocy to chemistry staining showed that the protein expressions of CD44、CD29 were detected in cells we isolated; chondrogenic, osteogenic, and adi pogenic differentiation assays were used to demonstrate the multi-differentiation potential of the TSPCs; the m RNA expressions of collagen type I and Tenascin-C were 5.3 and 2.1 times higher than that in cells with no differentiation（P〈0.01）, the protein expressions of tenascin C and collagen type I were 3.3 and 1.8 times higher than that in cells with no differentiation（P〈0.05）.Conclusion： Connective tissue growth factor（CTGF）can be used to promote tenogenic differentiation of the TSPCs？ in vitro.
出处
《湖北体育科技》
2015年第3期217-220,共4页
Hubei Sports Science
基金
北京市青年英才计划(YETP0407)
教育部中央高校基本科研业务费(FRFBR13038)
关键词
体育运动
肌腱
干细胞
分化
结缔组织
生长因子
sports
muscle tendon
stem cells
differentiation
connective tissue
growth factor
