摘要
目的:观察富血小板纤维蛋白(platelet-rich fibrin,PRF)复合自体第3代骨髓间充质干细胞(bone marrow stem cells,BMSCs)修复牙槽骨缺损的能力。方法:取健康雄性2月龄新西兰兔36只,随机分为4组:PRF+BMSCs组、PRF组、BMSCs组、空白对照组,均在全麻下微创拔除下颌左侧中切牙。4组实验动物分别在4、8、12周处死。取其下颌骨进行牙槽骨标本测量,X线观察,组织学观察。结果:牙槽骨标本测量比较:4组牙槽嵴宽度、高度丧失值均存在差异(P<0.05);析因分析示PRF与BMSCs复合使用修复牙槽骨的效果优于单独使用PRF或BMSCs(P<0.05)。X线、组织学观察表明术后4、8、12周时拔牙窝骨吸收程度:PRF+BMSCs组<PRF组<BMSCs组<空白对照组,PRF+BMSCs组成骨情况均优于PRF组、BMSCs组、空白对照组。结论:PRF复合自体BMSCs可以促进牙槽骨的再生与修复,具有潜在的临床应用价值。
Objective：To observe the bone formation of platelet-rich fibrin（PRF）combined with autogenous bone marrow stem cells（BMSCs）in rabbits alveolar bone defects.Methods：Thirty-six male 2months rabbits were divided into four groups randomly：PRF＋BMSCs,PRF,BMSCs,blank control.They were minimally invasive removal of mandible incisor under general anesthesia on the left side.After 4 weeks,8 weeks,12 weeks,36 rabbits were sacarificed by injecting air to vein.Then the jaws were investigated by general observation,X-ray photography and structual observation.Results：There were significant difference in alveolar ridge width and height among four groups via measuring alveolar bone specimen.The effect of repairing alveolar bone in BMSCs＋PRF was better than using BMSCs or PRF alone in factorial analysis（P〈0.05）.X-ray and histological observation showed that the degree of bone resorption was observed as follows：PRF＋BMSCs〈PRF〈BMSCs〈blank control（P〈0.05）and the bone formation of PRF＋BMSCs was better than PRF,BMSCs and blank control after 4weeks,8weeks and 12 weeks.Conclusion：PRF composited BMSCs can promote alveolar bone regeneration,repairation and had potential clinical application value.
出处
《口腔医学研究》
CAS
CSCD
北大核心
2015年第4期336-339,共4页
Journal of Oral Science Research
基金
新疆维吾尔自治区青年科学基金项目(编号:2013211B53)
