摘要
目的:探讨骨组织形成过程中骨钙素表达规律,评价金刚石-钛涂膜,纯钛和羟基磷灰石三种材料植入界面骨细胞功能情况,以利筛选出新的种植材料。方法:本实验将金刚石-钛涂膜、纯钛和羟基磷灰石三种种植材料,植入鼠下颌骨内,进行骨界面骨钙素mRNA检测及骨钙素免疫组织化学染色。结果:金刚石-钛涂膜材料新骨生成启动早,能明显加快组织的骨性愈合;金刚石和羟基磷灰石种植材料骨钙素mRNA表达量明显大于纯钛(P<0.01)。结论:骨钙素mRNA表达与界面区细胞增殖、新骨形成有关;骨钙素mRNA的检测有助于界面区细胞功能状态的判断。
Objective：To get the expression rule of osteocalcin during the formation of bone tissue. To evaluate the cell function of interface of three different materials, to get the new implant materials. Methed： three different implant materials ： chemical-vapor-deposited diamond（CVD） , pure titanium （Ti） and hydroxyapatite （HA） were employed into the mandibular bone of Wistar rats. Osteocalcin mRNA was tested and immunohistochemical staining of osteocalcin was done. Result： the formation of new bone is more active and earlier in CVD diamond film than in HA and Ti materials. CVD diamond film can expedite osteointegration; the expression of Osteocalcin mRNA in the CVD and HA is much more significant than in Ti （P 〈 0.01 ）. Conclusion： expression of Osteocalcin mRNA is correlated with cell increment of interface and new bone formation; Ostcocalcin mRNA examination is helpful to judge the cell function status in the interface.
出处
《临床口腔医学杂志》
2006年第1期17-19,共3页
Journal of Clinical Stomatology
