摘要
目的探讨人参皂甙RD对大鼠蛛网膜下腔出血(subarachnoid hemorrhage,SAH)后脑血管痉挛(cerebral vasospasm,CVS)的防治作用及作用机制。方法 48只SD大鼠随机分为穿刺组、模型组、人参皂苷RD组和丙二醇组,每组12只;模型组、人参皂苷RD组和丙二醇组采用枕大池二次注血法建立大鼠SAH模型,穿刺组仅行枕大池穿刺而不注血,模型组造模后无干预措施,人参皂苷RD组和丙二醇组造模后分别腹腔注射人参皂甙RD稀释液和丙二醇,1次/d;造模5d后行神经行为学评分,采用HE染色和透射电镜观察基底动脉形态和超微结构改变。结果人参皂苷RD组神经行为学评分(3.75±0.62)明显高于模型组(3.17±0.72)和丙二醇组(3.25±0.45),低于穿刺组(4.92±0.29)(P<0.05);人参皂苷RD组基底动脉管壁厚度((20.03±0.44)μm)明显小于模型组((25.12±0.37)μm)和丙二醇组((25.06±0.38)μm),大于穿刺组((16.89±0.41)μm)(P<0.05),管腔面积((36.39±1.41)×103μm2)明显大于模型组((21.20±0.86)×103μm2)和丙二醇组((21.41±1.03)×103μm2),小于穿刺组((55.09±1.20)×103μm2)(P<0.05);人参皂苷RD组基底动脉超微结构病理性改变减轻。结论非电压依赖性钙通道拮抗剂人参皂甙RD可在一定程度上缓解CVS。
Objective To investigate the effect of ginsenoside-Rd on prevention and treatment of cerebral vasospasm after subarachnoid hemorrhage and its mechanism.Methods Forty-eight male SD rats were randomly divided into puncture group,model group,ginsenoside-Rd group and propylene glycol group,with 12 rats in each group.The SAH rat models were established by injecting autologous arterial blood into the cistern magna twice in model group,ginsenoside-Rd group and propylene glycol group.Puncture group was puntured but was not injected blood.Ginsenoside-Rd group and propylene glycol group were injected intraperitoneally ginsenoside-Rd and propylene glycol respectively once a day.Model group was not intervened after the establishment of models.The neurological behavior score was measured on the fifth day after subarachnoid hemorrhage,and HE staining and transmission electron microscope were used to observe the pathological changes of the basilar artery.Results The neurological behavior score in ginsenoside-Rd group（3.75±0.62）was significantly higher than that in model group（3.17±0.72）and propylene glycol group（3.25±0.45）,but lower than that in puncture group（4.92±0.29）（P〈0.05）.The wall thicknesses of basilar artery in ginsenoside-Rd group（（20.03±0.44）μm）was significantly thinner than that in model group（（25.12±0.37）μm）and propylene glycol group（25.06±0.38）μm）,but thicker than that in puncture group（（16.89±0.41）μm）（P〈0.05）.The lumen area of basilar artery in ginsenoside-Rd group（（36.39±1.41）×103μm2）was significantly larger than that in model group（（21.20±0.86）×10^3μm^2）and propylene glycol group（（21.41±1.03）×10^3μm^2）,but smaller than that in puncture group（（55.09±1.20）×10^3μm^2）（P〈0.05）.The ultrastructural pathological changes in the basilar artery were found in ginsenoside-Rd group.Conclusion Ginsenoside-Rd,a blocker of non-voltage-dependent calcium channel,could alleviate cerebral vasospasm to some extent.
出处
《中华实用诊断与治疗杂志》
2015年第4期348-351,共4页
Journal of Chinese Practical Diagnosis and Therapy
基金
国家自然科学基金(30870844)
