摘要
目的:探讨阿托伐他汀对大鼠蛛网膜下腔出血(SAH)后脑血管痉挛(CVS)的缓解效果及对线粒体融合蛋白2(Mitofusin-2)、脑源性神经营养因子(BDNF)表达的影响,为SAH后CVS的防治提供实验依据和新方法。方法:选取雄性SD大鼠30只,随机数字表法随机分为假手术组、模型组和治疗组,每组10只。模型组和治疗组使用枕大池二次注血法制作蛛网膜下腔出血模型,假手术组同样操作方式注入生理盐水。治疗组予阿托伐他汀20 mg/kg溶于2 mL蒸馏水灌胃。假手术组与模型组给与蒸馏水2 mL灌胃。观察干预5 d后各组大鼠的体重、死亡率、神经功能缺失情况、基底动脉血管内径、管壁厚度以及平滑肌细胞凋亡情况,观察各组Mitofusin-2、BDNF表达水平。结果: 3组大鼠的体重由低到高分别为假手术组、治疗组、模型组,差异具有统计学意义( P <0.05),假手术组和治疗组各有1只大鼠死亡,模型组有2只大鼠死亡,3组死亡率无显著差异( P >0.05)。3组神经功能评分由低到高分别为模型组、治疗组和假手术组,差异具有统计学意义( P <0.05)。3组血管内径由小到大分别为模型组、治疗组、假手术组,差异具有统计学意义( P <0.05),而血管壁的厚度由小到大分别为假手术组、治疗组、模型组,差异具有统计学意义( P <0.05)。3组血管内皮细胞凋亡率由低到高分别为模型组、治疗组和假手术组,差异具有统计学意义( P <0.05)。Mitofusin-2的表达水平由低到高分别为假手术组、模型组和治疗组,差异具有统计学意义( P <0.05),BDNF的表达水平由低到高分别为假手术组、治疗组和模型组,差异具有统计学意义( P <0.05)。结论:阿托伐他汀能够减轻SAH后CVS的发生,减轻脑组织的损伤,且其机制可能通过上调Mitofusin-2的表达相关。
Objective: To investigate the alleviating effect of atorvastatin on CVS after SAH in rats and its effect on the expression of Mitofusin-2 and BDNF,so as to provide experimental basis and new methods for the prevention and treatment of CVS after SAH. Methods : Thirty SPF male SD rats were randomly divided into sham operation group,model group and treatment group with 10 rats in each group.Subarachnoid hemorrhage model was made by occipital cistern twice injection in model group and treatment group.Normal saline was injected into sham operation group in the same way.The treatment group was given atorvastatin 20 mg/kg dissolved in 2 mL distilled water by gavage.Sham operation group and model group were given 2 mL distilled water by gastric perfusion.The weight,mortality,neurological deficits,vascular diameter,wall thickness and apoptosis of smooth muscle cells in basal artery of rats in each group were compared,and the expression levels of Mitofusin-2 and BDNF in each group were observed.Result: The body weight of three groups of rats from low to high was sham operation group,treatment group and model group ( P < 0.05).One rat died in the sham operation and the treatment group.Two rats died in the model group,and there was no significant difference in mortality among the three groups (P > 0.05).The scores of neurological function of the three groups from low to high were model group,treatment group and sham operation group ( P < 0.05).The diameter of blood vessel in three groups from small to large were model group,treatment group and sham operation group,the difference was statistically significant ( P < 0.05),while the thickness of blood vessel wall from small to large were sham operation group,treatment group and model group,the difference was statistically significant ( P < 0.05).The apoptotic rate of vascular endothelial cells in the three groups from low to high were model group,treatment group and sham operation group,with statistical significance ( P < 0.05).The expression levels of Mitofusin-2 in sham-opera
作者
侯鹏飞
刘展会
张睿
舒庆
程文佳
HOU Peng-fei;LIU Zhan-hui;ZHANG Rui;SHU Qing;CHENG Wen-jia(Department of Neurosurgery,Ninth Hospital of Xi'an City,Xi'an,Shaanxi Province,710054;Conversion Medical Center of Xi'an Ninth Hospital,Xi'an,Shaanxi Province,710054;Department of Pathology,Ninth Hospital of Xi'an City,Xi'an,Shaanxi Province,710054)
出处
《海南医学院学报》
CAS
2019年第13期965-969,共5页
Journal of Hainan Medical University
基金
陕西省重点研发计划项目(2018SF-093)~~
